The therapeutic potential of truffle fungi: a patent survey

Małgorzata Gajos, Florian Ryszka, Joerg Geistlinger


The purpose of this article is to research and retrieve patent information regarding the therapeutic use of truffles. Truffles have a unique value as a foodstuff and impact positively on human health and well-being. They are applied in such industries as the pharmaceutical industry and the cosmetic industry. Patent documentation available in the Espacenet network and the Patentscope service were analyzed by key word and patent specifications were examined to describe state of the art and to identify scientific research trends in therapeutic applications of truffles. Medicinal properties of truffles such as the anticancer or cardiovascular effect, a reduction in blood lipids, immunological resistance and increased energy were identified. Other therapeutic benefits include sedative action, prevention of hormonal imbalances in women, pre-menopause symptom relief, senile urethritis and prostate disorders, sleep disorders and increased absorption of calcium from milk. Truffles can also be used to alleviate symptoms of milk intolerance such as diarrhoea or bloating, to ease rheumatic pains and to treat and prevent further development or recurrence of senile cataract.


truffle fungi; Tuber; therapeutic potential of truffles; patent survey

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Intellectual Property Statistics Data Center [Internet]. 2014 [cited 2014 Feb 20]; Available from:

Gajos M, Hilszczańska D. Research on truffles: Scientific journals analysis. Scientific Research and Essays 2013;8(38):1837 1847.

Hannan MA, Al-Dakan AA, Aboul-Enein HY, Al-Othaimeen AA. Mutagenic and antimutagenic factor(s) extracted from a desert mushroom using different solvents. Mutagenesis. 1989;4(2):111–114.

Murcia MA, Martinez-Tomé M, Jiménez AM, Vera AM, Honrubia M, Parras P. Antioxidant activity of edible fungi (truffles and mushrooms): losses during industrial processing. J Food Prot. 2002;65(10):1614 1622.

Al-Laith AAA. Antioxidant components and antioxidant/antiradical activities of desert truffle (Tirmania nivea) from various Middle Eastern origins. J Food Compost Anal. 2010;23(1):15–22.

Amicarelli F, Bonfigli A, Colafarina S, Cimini AM, Pruiti B, Cesare P, et al. Glutathione dependent enzymes and antioxidant defences in truffles: organisms living in microaerobic environments. Mycol Res. 1999;103(12):1643–1648.

Villares A, García-Lafuente A, Guillamón E, Ramos Á. Identification and quantification of ergosterol and phenolic compounds occurring in Tuber spp. truffles. J Food Compost Anal. 2012;26(1–2):177–182.

Janakat S, Nassar M. Hepatoprotective activity of desert truffle (Terfezia claveryi) in comparison with the effect of Nigella sativa in the rat. Pakistan Journal of Nutrition. 2010;9(1):52 56.

Janakat S, Al-Fakhiri S, Sallal AK. A promising peptide antibiotic from Terfezia claveryi aqueous extract against Staphylococcus aureus in vitro. Phytother Res. 2004;18(10):810 813.

Janakat SM, Al-Fakhiri SM, Sallal AKJ. Evaluation of antibacterial activity of aqueous and methanolic extracts of the truffle Terfezia claveryi against Pseudomonas aeruginosa. Saudi Med J. 2005;26(6):952–955.

Nazzaro F, Fratianni F, Picariello G, Coppola R, Reale A, Luccia AD. Evaluation of gamma rays influence on some biochemical and microbiological aspects in black truffles. Food Chem. 2007;103(2):344–354.

Hussan G, Al-Ruqaie IM. Occurrence in chemical composition, and nutritional value of truffles: overview. Pak J Biol Sci. 1999;2(2):510 514.

Fratianni F, Di Luccia A, Coppola R, Nazzaro F. Mutagenic and antimutagenic properties of aqueous and ethanolic extracts from fresh and irradiated Tuber aestivum black truffle: a preliminary study. Food Chem. 2007;102(2):471 474.

Stanikunaite R, Khan SI, Trappe JM, Ross SA. Cyclooxygenase-2 inhibitory and antioxidant compounds from the truffle Elaphomyces granulatus. Phytother Res. 2009;23(4):575 578.

Tang YJ, Zhu LL, Liu RS, Li HM, Li DS, Mi ZY. Quantitative response of cell growth and Tuber polysaccharides biosynthesis by medicinal mushroom Chinese truffle Tuber sinense to metal ion in culture medium. Bioresour Technol. 2008;99(16):7606 7615.

Ferreira ICFR, Vaz JA, Vasconcelos MH, Martins A. Compounds from wild mushrooms with antitumor potential. Anticancer Agents Med Chem. 2010;10(5):424–436.

Ng TB, Wong JH. Fungal proteins with antiproliferative and anticancer activities. Protein Pept Lett. 2013;20(4):433–438.

Jinming G, Lin H, Jikai L. A novel sterol from Chinese truffles Tuber indicum. Steroids. 2001;66(10):771–775.

Martin F, Kohler A, Murat C, Balestrini R, Coutinho PM, Jaillon O., et al. Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis. Nature. 2010;464:1033 1038.

Murat C, Riccioni C, Belfiori B, Cichocki N, Labbé J, Morin E, et al. Distribution and localization of microsatellites in the Perigord black truffle genome and identification of new molecular markers. Fungal Genet Biol. 2011;48(6):592 601.

Lacourt I, Duplessis S, Abbà S. Isolation and characterization of differentially expressed genes in the mycelium and fruiting body of Tuber borchii. J Appl Environ Microbiol. 2002;68(9):4574 4582.

Splivallo R, Ottonello S, Mello A, Karlovski P. Truffle volatiles: from chemical ecology to aroma biosynthesis. New Phytol. 2011;189(3):688 699.

Bolchi A, Ruotolo R, Marchini G, Vurro E, di Toppi LS, Kohler A, et al. Genome-wide inventory of metal homeostasis-related gene products including a functional phytochelatin synthase in the hypogeous mycorrhizal fungus Tuber melanosporum. Fungal Genet Biol. 2011;48(6):573 584.

Navarro-Ródenas A, Ruíz-Lozano JM, Kaldenhoff R, Morte A. The Aquaporin TcAQP1 of the desert truffle Terfezia claveryi is a membrane pore for water and CO2 transport. Mol Plant Microbe Interact. 2012;25(2):259 266.

Tang Y, Li HM, Tang YJ. Comparison of sterol composition between Tuber fermentation mycelia and natural fruiting bodies. Food Chem. 2012;132(3):1207 1213.

White MJ. esp@cenet Europe’s Network of Patent Databases. Issues Sci Technol Lib. 2006;47.

Gajos M. Opis patenowy jako źródło informacji. Katowice: University of Silesia; 2000.

Handbook on industrial property information and documentation [Internet]. 2013 [cited 2013 Dec 19]; Available from:

International patent classification – version 2013. Guide [Internet]. 2013 [cited 2013 Dec 15]; Available at: