Isolation and molecular identification of laccase-producing saprophytic/phytopathogenic mushroom-forming fungi from various ecosystems in Michoacán State, Mexico

Irum Mukhtar, Marina Arredondo-Santoyo, Ma. Soledad Vázquez-Garcidueñas, Gerardo Vázquez-Marrufo

Abstract


The aim of this study was isolation and molecular identification of laccase-producing saprophytic/phytopathogen Basidiomycetes species from different geographic regions with dominant vegetation of Pinus, Abies, and Quercus spp. in the state of Michoacán, Mexico. Soil samples and visible mycelial aggregates were collected for fungal isolations. Soil samples were processed using a soil particle washing technique, where a selective Ascomycetes inhibitor and guaiacol, as an indicator of saprophytic Basidiomycetes growth, were used. Most of the isolates were obtained from samples collected in Parque Nacional, José Ma. Morelos (Km 23), Charo, Michoacán, Mexico. Based on sequence comparisons and phylogenetic analysis of internal transcribed spacer regions (ITS1-5.8S-ITS4) with respect to reference taxa, identification of saprophytic/phytopathogen Basidiomycetes species was carried out. In total, 15 isolates from 12 genera (i.e., Bjerkandera, Coriolopsis, Ganoderma, Hexagonia, Irpex, Limonomyces, Psathyrella, Peniophora, Phlebia, Phlebiopsis, Trametes, and Trichaptum) and one species from family Corticiaceae were identified. This study will be useful for further investigations on biodiversity of soil Basidiomycetes in different ecosystems. At present, these isolates are being used in our various lab experiments and can be useful in different industrial and bioremediation applications.

Keywords


Basidiomycetes; guaiacol; geographic regions; saprophytic

Full Text:

PDF

References


Rayner A, Boddy L. Fungal decomposition of wood. New York, NY: John Wiley & Sons; 1988.

Sette LD, Oliveira VM, de Rodrigues MFA. Microbial lignocellulolytic enzymes: industrial applications and future perspectives. Microbiol Aust. 2008;29:8–20.

Desai SS, Nityanand C. Microbial laccases and their applications: a review. Asian Journal of Biotechnology. 2011;3:98–124. https://doi.org/10.3923/ajbkr.2011.98.124

Mittermeier RA, Mittermeier CG. La importancia de la Diversidad Biológica de México. In: Sarukan J, Dirzo R, editors. México ante los retos de la Biodiversidad. México: CONABIO; 1992.

Thorn RG, Reddy CA, Harris D, Paul EA,. Isolation of saprophytic Basidiomycetes from soil. Appl Environ Microbiol. 1996;62:4288–4292.

Arora DS, Sandhu DK. Survey of some Indian soils for laccase producing fungi and their lignin degrading ability. Proceedings of Indian Academy of Science, Plant Sciences. 1985;94:567–574.

Nishida T, Kashino Y, Mimura A, Takahara Y. Lignin biodegradation by wood-rotting fungi. Mokuzai Gakkaishi. 1988;34:530–536.

White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols – a guide to methods and applications. San Diego, CA: Academic Press; 1990. p. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar, S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28(10):2731–2739. https://doi.org/10.1093/molbev/msr121

Hutchison LJ. Studies on the systematics of ectomycorrhizal fungi in axenic culture. III. Patterns of polyphenol oxidase activity. Mycologia. 1990;82:424–435. https://doi.org/10.1080/00275514.1990.12025905

Machado KMG, Matheus DR, Bononi VLR. Ligninolytic enzymes production and remazol brilliant blue R decolorization by tropical Brazilian Basidiomycetes fungi. Brazilian Journal of Microbiology. 2005;36:246–252. https://doi.org/10.1590/S1517-83822005000300008

Kiiskinen LL, Rättö M, Kruus K. Screening for novel laccase-producing microbes. J Appl Microbiol. 2004;97(3):640–646. https://doi.org/10.1111/j.1365-2672.2004.02348.x

Masalu R. Lignin degrading enzymes from mycelial cultures of wild Tanzanian mushrooms [Master thesis]. Tanzania: University of Dar es Salaam; 2004.

More SS, Renuka PS, Pruthvi K, Swetha M, Malini S, Veena SM. Isolation, purification, and characterization of fungal laccase from Pleurotus sp. Enzyme Res. 2011;2001:248735. https://doi.org/10.4061/2011/248735

Poojary H, Hoskeri A, Kaur A, Mugeraya G. Comparative production of ligninolytic enzymes from novel isolates of Basidiomycetes and their potential to degrade textile dyes. Nat Sci. 2012;10:90–96.

Singh N, Abraham J. Isolation of laccase producing fungus from compost soil and partial characterization of laccase. Advances in Applied Science Research. 2013;4(5):91–98.




DOI: https://doi.org/10.5586/am.1119

Journal ISSN:
  • 2353-074X (online)
  • 0001-625X (print; ceased since 2015)
This is an Open Access journal, which distributes its content under the terms of the Creative Commons Attribution License, which permits redistribution, commercial and non-commercial, provided that the content is properly cited.
The journal is a member of the Committee on Publication Ethics (COPE) and aims to follow the COPE’s principles.
The journal publisher is a member of the Open Access Scholarly Publishers Association.
The journal content is indexed in Similarity Check, the Crossref initiative to prevent scholarly and professional plagiarism.
Publisher
Polish Botanical Society