Abstract
Studies were carried out to determine the effect of vitamins, temperature and pH on the biomass production by ectomycorrhizal fungi: Laccuria bicolor, Hebeloma crustuliniforme, Suillus bovinus and Pisolithus arhizus. Responses of particular organisms were different Three-factor ANOVA which enables the comparison of the effect of experimental factors on biomass yield has shown that the pH of the medium had a stronger effect on L. bicolor, H. crustuliniforme and S. bovinus than vitamins. Temperature was the factor exerting the strongest effect on the growth of P. tinctorius.