A simple rapid method for protein determination

Janusz Czapski


A simple, rapid method is described for determination of protein in the range of 20-200 µg. The procedure is based on the reaction between protein and amido black 10B. An insoluble residue is precipitated and the dye concentration in solution decreases. The difference between the optical density of the blank (without protein) and sample with protein is a linear function of protein content.

Full Text: