Rozmnażanie Platanus × acerifolia Willd. i Salix alba L. cv. 'Tristis' metodą kultur tkankowych in vitro [Reproduction of Platanus × acerifolia Willd. and Salix alba L. cv. 'Tristis' by tissue culture in vitro]

Abstract

The experiments were performed to establish the conditions of Platanus × acerifolia Willd. and Salix alba L. cv. 'Tristis' reproduction by tissue culture. Two experiments were run. Leaf buds of Platanus and inflorescence buds of Salix were used. Growth of callus from Platanus and Salix in dependence on the concentration of growth substances (BA and NAA) and light and darkness conditions were compared. Rooted explants of Salix alba L. cv. 'Tristis' were obtained. The best results were obtained with Platanus × acerifolia in which rhizogenesis of amorphic callus occurred after two months of the experiment. Darkness was not a necessary condition for root formation. After three months of the experiment completely developed Platanus × acerifolia plants were obtained composed of a foliated shoots and a bundle of roots. They could be planted out on a sterile permanent substrate.