Patogeniczność izolatów Fusarium avenaceum (Fr.) Sacc. w stosunku do liści tulipana, oceniana w teście na krążkach z liści [Pathogenicity of Fusarium avenaceum isolates to tulip leaves assessed on leaf disks]

Abstract

The purpose of this study was to investigate the pathogenicity of 14 Fusarium avenaceum (Giberella avenacea) isolates, obtained from tulip plantations located in Puławy region, to leaves of tulip cv. Apeldoorn. Mycelial plugs 3 mm in diameter from the margins of the colonies growing on PDA and SNA were placed on 20 mm disks cut from tulip leaves. The disks were placed abaxial surface up, in Petri dishes on top of filter paper soaked with 100 mg/l gibberellic acid to retard leaf senescence. There were 5 replications for each isolate. Leaves in each Petń dish were inoculated with one isolate. Plates were incubated at 20°C and lesion diameters were measured after 4 days. The pathogenicity of investigated isolates was compared by measuring lesion development on tulip leaves. All of isolates colonized leaves disks succesfull – causing after 4 days necrotic lesions and sporulating after 14 days what indicated that an infection had occurred. There were significant differences in lesions size among isolates. Isolates: 27, 1, 2, 48 and 3 caused largest lesions at mean size from 150 mm² to 163 mm² what indicates their high pathogenicity. Less pathogenic were isolates: 28, 42, 7 and 72 caused lesions at mean size from 46 mm² to 97 mm². On average, mycelial plugs taken from PDA medium colonized leaf disks more efficient in comparison to them taken from SNA, that caused smaller lesions.