Use of tetrazolium (TTC), Germ's and greenhouse plant emergences methods for testing seed vigour of selected ornamental plant species

Roman Hołubowicz, Xian Wang, Wiesław Siutaj

Abstract


In the years 1996-1997 the experiments were carried out on methods to investigate seed vigour of tassel flower (Amaranthus caudatus L.), sand pink (Dianthus chinensis L.), babies' breath (Gypsophila elegans M.B.), sweet pea (Lathyrus odorathus L.), African marigold (Tagetes erecta L.) and zinnia (Zinnia elegans Jasq.). The main goals of this research were to specify conditions for accelerated ageing (AA) of the seeds of a few selected ornamental plant species and to choose the most appropriate methods for their seed vigour evaluation in the laboratory and greenhouse conditions. All used in the experiments seeds came from the commercial seed lots from Polish seed company. Evaluation was carried out on the seed samples with high and low vigour. The latter ones were received through subjecting the seed samples to AA, i.e. by placing them in 100% relative humidity (RH) at 44°C, except African marigold-at 42°C, in the darkness and keeping them for 144, 88, 100, 48, 72 and 72 hours, respectively. The tested seed vigour estimated methods included the Germ's method, the 2,3,5-triphenyl tetrazoilum chloride (TTC) method and the test of plant emergences in the greenhouse. The high vigour seeds samples were used as a check. The Germ's method was found to be useful to evaluate sand pink, babies' breath and African marigold seed vigour, whereas the TTC method was found to be suitable for vigour evaluation of sand pink, babies' breath and zinnia. At present stage of our knowledge about seed vigour, the plant emergences in the greenhouse method was found to be the best for evaluation of seed vigour of tassel flower, sand pink, babies' breath, sweet pea and zinnia. It is reasonable to combine a few methods of seed vigour evaluation for ornamental plant species.

Keywords


seed vigour; tassel flower; sand pink; babies' breath; sweet pea African marigold; zinnia; Amaranthus caudatus; Dianthus chinensis; Gypsophila elegans; Lathyrus odorathus; Tagetes erecta; Zinnia elegans; tetrazo|ium method; Germ's method; accelerated ageing test

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