Plant regeneration of Alstroemeria in vitro

Abstract

The regenerative ability of explants from various organs of Alstroemeria plants was investigated. Rhizome apical and axillary tips cultured on the Murashige and Skoog medium with BA - 2. mgl^-1 and NAA - 0,5 mgl^-1 were the best among the tissue tested as initial explants. Five weeks after isolation the rhizome with 1-4 upright growing shoots were obtained. The types of rhizome explants influenced development and growth of lateral rhizomes and upright growing shoots. There were no significant differences in number of roots formed on various kind of rhizome explants. Rooting was strongly influenced by NAA. Subapical segments of vegetative stem, segments of flower pedicels and parts of ovary did not regenerate rhizome or roots but occasionally callus was formed on the medium with kinetin - 2 mgl^-1 and NAA - 2 mgl^-1. Segments excised from vegetative stem sporadically developed roots on the medium with NAA or IBA in concentrations 3 and 9 mgl^-1.