Identyfikacja wybranych gatunków grzybów z rodzaju Fusarium z nasion niektórych gatunków roślin uprawnych metodą tradycyjną i BIO-PCR [Identification of some Fusarium species from selected crop seeds using traditional method and BIO-PCR]

Tomasz Kulik, Gabriel Fordoński, Agnieszka Pszczółkowska, Krystyna Płodzień, Jacek Olszewski

Abstract


We identified a species level of the fungal cultures isolated from selected crop seeds using traditional method and BIO-PCR. The use of BIO-PCR did not correspond completely to the morphological analyses. Both methods showed increased infection with F. poae in winter wheat seed sample originated from north Poland. Fungal culture No 40 (isolated from faba bean and identified with traditional method as mixed culture with F. culmorum and F. graminearum) did not produce expected product after PCR reaction with species specific primers OPT18F470, OPT18R470. However, the use of additional primers Fc01F, Fc01R allowed for reliable identification of F. culmorum in the culture.

Keywords


Fusarium spp.; identyfikacja; BIO-PCR; SCAR

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