Identification of three species commonly known as “daqingye” by internal leaf anatomy and high-performance liquid chromatography analyses

Yingjiao Zhang, Yongqing Zhu, Jian Chen, Chen Xia, Junling Deng, Huajia Li, Yanling Li, Juan Li, Pei Liu

Abstract


The macroscopic and microscopic morphologies and indigo and indirubin concentration of the traditional Chinese medicine herbs Isatis indigotica Fort., Polygonum tinctorium Ait., and Baphicacanthus cusia (Nees) Bremek, all commonly known as “daqingye”, were determined and compared. The morphological analyses indicated that I. indigotica has leaves with winged petioles and no glandular hairs or crystals, P. tinctorium has leaves with membranous ocrea and clusters of calcium oxalate, and B. cusia has palisade cells in the mesophyll running over the main vein and single cells containing calcium carbonate crystals. Indigo and indirubin are chemical constituents that have been previously isolated from daqingye and were selected in this study as identification markers for high-performance liquid chromatography analysis due to their pharmacological activities. The chromatographic results showed that indigo and indirubin concentration varied significantly among the three species: high concentration of both indigo and indirubin were observed in I. indigotica, the highest concentration among the three daqingye plants was found in P. tinctorium but with low levels of indirubin, and the concentration of indigo and indirubin was quite low in B. cusia. In summary, three different species commonly known as daqingye were accurately distinguished by morphological observation, internal leaf anatomy analysis, and chromatographic analysis.

Keywords


daqingye; Isatis indigotica; Polygonum tinctorium; Baphicacanthus cusia (Nees) Bremek; internal leaf anatomy; high-performance liquid chromatography

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References


Zhang X, editor. General guidelines for methodologies on research and evaluation of traditional medicine. Geneva: World Health Organization; 2000.

But PP. Herbal poisoning caused by adulterants or erroneous substitutes. J Trop Med Hyg. 1994; 97(6):371–374.

Pittler MH, Ernst E. Systematic review: hepatotoxic events associated with herbal medicinal products. Aliment Pharmacol Ther. 2003;18(5):451–471. https://doi.org/10.1046/j.1365-2036.2003.01689.x

Xiaq M, Shi Q, Duan JA, Tina T, Dong X, Karl W, et al. Chemical analysis of Radix Astragali (Huangqi) in China: a comparison with its adulterants and seasonal variations. Agricultural and Food Chemistry. 2002;50(17):4861–4866. https://doi.org/10.1021/jf0202279

Zhao Z, Hu Y, Liang Z, Yuen JP, Jiang Z, Leung KS. Authentication is fundamental for standardization of Chinese medicines. Planta Med. 2006;72(10):865–874. https://doi.org/10.1055/s-2006-947209

Yanbo Z, Pangchui S, Chowing S, Zhengtao W, Yao T. Molecular authentication of Chinese herbal materials. J Food Drug Anal. 2007;15(1):1–9.

Phua DH, Zosel A, Heard K. Dietary supplements and herbal medicine toxicities – when to anticipate them and how to manage them. Int J Emerg Med. 2009;2(2):69–76. https://doi.org/10.1007/s12245-009-0105-z

Joshi VC, Avula B, Khan IA. Authentication of Stephania tetrandra S. Moore (Fang Ji) and differentiation of its common adulterants using microscopy and HPLC analysis. J Nat Med. 2008;62(1):117–121. https://doi.org/10.1007/s11418-007-0200-5

Wosch L, Imig DC, Cervi AC, Moura BB, Budel JM, Santos CAM. Comparative study of Passiflora taxa leaves. I. A morpho-anatomic profile. Revista Brasileira de Farmacognosia. 2015;25(4):328–343. https://doi.org/10.1016/j.bjp.2015.06.004

Mingli S, Xiaowei M, Zhen W, Wenming Y, Xingnian C, Dequan Y, et al., editors. Pharmacopoeia of the People’s Republic of China. Beijing: China Medical Science Press; 2010.

Tang W, Eisenbrand G. Qingdai. In: Tang W, Eisenbrand G. Chinese drugs of plant origin: chemistry, pharmacology, and use in traditional and modern medicine. Berlin: Springer; 1992. p. 805–812. https://doi.org/10.1007/978-3-642-73739-8_103

Hamburger M. Isatis tinctorial – from the rediscovery of an ancient medicinal plant towards a novel anti-inflammatory phytopharmaceutical. Phytochem Rev. 2002;1(3):333–344. https://doi.org/10.1023/A:1026095608691

Brand E, Leon C, Nesbitt M, Guo P, Huang R, Chen H, et al. Economic botany collections: a source of material evidence for exploring historical changes in Chinese medicinal materials. J Ethnopharmacol. 2017;200:209–227. https://doi.org/10.1016/j.jep.2017.02.028

Liu X, Fang K, Pan J, Wang Y. Quantitative analysis of 4(3)-quinazolinedione in 4 “Daqingye”, leave of Isatis indigotica, Baphicacanthus cusia, Polygonum tinctorium and Clerodendron cyrtophyllum. Journal of Chinese Medicinal Materials. 2000;23(7):388–389. https://doi.org/10.13863/j.issn1001-4454.2000.07.009

Xiao Z, Hao Y. From Danggui Longhui Wan to meisoindigo: experience in the treatment of chronic myelogenous leukemia in China. In: Meijer L, Guyard N, Skaltousnis LA, Eisenbrand G, editors. Indirubin, the red shade of indigo. France: Life in Progress; 2006. p. 203–208.

Franzblau SG, Cross C. Comparative in vitro antimicrobial activity of Chinese medicinal herbs. J Ethnopharmacol. 1986;15(3):279–288. https://doi.org/10.1016/0378-8741(86)90166-2

Cooksey CJ. An annotated bibliography of recent significant publications on indigo and related compounds. Biotech Histochem. 2012;87(7):439–463. https://doi.org/10.3109/10520295.2012.698308

Liau BC, Jong TT, Lee MR, Chen SS. LC-APCI-MS method for detection and analysis of tryptanthrin, indigo, and indirubin in Daqingye and Banlangen. J Pharm Biomed Anal. 2007;43(1):346–351. https://doi.org/10.1016/j.jpba.2006.06.029

Boneberger S, Rupec RA, Ruzicka T. Complementary therapy for atopic dermatitis and other allergic skin diseases: facts and controversies. Clin Dermatol. 2010;28(1):57–61. https://doi.org/10.1016/j.clindermatol.2009.03.017

Reuter J, Woelfle U, Weckesser S, Schempp C. Which plant for which skin disease? Part 1: atopic dermatitis, psoriasis, acne, condyloma and herpes simplex. Journal Der Deutschen Dermatologischen Gesellschaft. 2010;8(10):788–796. https://doi.org/10.1111/j.1610-0387.2010.07496.x

Zhang H, Yang L, Liu S, Ren L. Study on active constituents of traditional Chinese medicine reversing multidrug resistance of tumor cells in vitro. Journal of Chinese Medicinal Materials. 2001;24(9):655–657. https://doi.org/10.3321/j.issn:1001-4454.2001.09.019

Eisenbrand G, Hippe F, Jakobs S, Muehlbeyer S. Molecular mechanisms of indirubin and its derivatives: novel anticancer molecules with their origin in traditional Chinese phytomedicine. J Cancer Res Clin Oncol. 2004;130(11):627–635. https://doi.org/10.1007/s00432-004-0579-2

Sugihara K, Yamada T, Kitamura S, Ohta S, Yamashita K, Okamura S, et al. Aryl hydrocarbon receptor-mediated induction of microsomal drug-metabolizing enzyme activity by indirubin and indigo. Biochem Biophys Res Commun. 2002;318(2):571–578. https://doi.org/10.1016/j.bbrc.2004.04.066

Kim MH, Choi YY, Yang G, Cho IH, Nam D, Yang WM. Indirubin, a purple 3,2-bisindole, inhibited allergic contact dermatitis via regulating T helper(Th)-mediated immune system in DNCB-induced model. J Ethnopharmacol. 2013;145(1):214–219. https://doi.org/10.1016/j.jep.2012.10.055

Karaman Ş, Diraz E, Çömlekçioğlu N, İlçim A, Durdu H, Tansi S. High yielding indigo sources in native Isatis (Brassicaceae) taxa from Turkey. Genet Resour Crop Evol. 2016;63(3):531–543. https://doi.org/10.1007/s10722-015-0269-8

Vuorema A, John P, Keskitalo M, Marken F. Electrochemical determination of plant-derived leuco-indigo after chemical reduction by glucose. J Appl Electrochem. 2008;38(12):1683–1690. https://doi.org/10.1007/s10800-008-9617-0

Wagner H, Bauer R, Melchart D, Xiao PG, Staudinge A, editors. Radix isatidis – Banlangen. In: Wagner H, Bauer R, Melchart D, Xiao PG, Staudinge A, editors. Chromatographic fingerprint analysis of herbal medicines. Vienna: Springer; 2011. p. 791–803. https://doi.org/10.1007/978-3-7091-0763-8_66

Kukula-Koch W, Koch W, Stasiak N, Głowniak K, Asakawa Y. Quantitative standarization and CPC-based recovery of pharmacologically active components from Polygonum tinctorium Ait. leaf extracts. Ind Crops Prod. 2015;69:324–328. https://doi.org/10.1016/j.indcrop.2015.02.048

Ruzin S. Plant microtechnique and microscopy. New York, NY: Oxford University Press; 1999.

Zheng JL, Wang MH, Yang XZ, Wu LJ. Study on bacteriostasis of Isatis indigotic Fort. Chinese Journal of Microecology. 2003;15(1):18–19. https://doi.org/10.3969/j.issn.1005-376X.2003.01.008

Chinese Pharmacopeia Commission. An illustrated handbook on microscopic identification of Chinese crude drugs for Chinese pharmacopoeia. Beijing: People’s Medical Publishing House; 2009.

Galal AM, Raman V, Avula B, Wang YH, Rumalla CS, Weerasooriya AD, et al. Comparative study of three Plumbago L. species (Plumbaginaceae) by microscopy, UPLC-UV and HPTLC. J Nat Med. 2013;67(3):554–561. https://doi.org/10.1007/s11418-012-0717-0

Guimarães A, Costa R, Cabral L, Vieira A. Comparative anatomy and chemical analysis of the vegetative organs of three species of Stigmaphyllon (Malpighiaceae). Flora. 2016;224:30–41. https://doi.org/10.1016/j.flora.2016.07.001




DOI: https://doi.org/10.5586/asbp.3575

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