Antioxidant activity, total phenolic content and flavonoid concentrations of different plant parts of Teucrium polium L. subsp. polium

Milan S. Stankovic, Neda Niciforovic, Vladimir Mihailovic, Marina Topuzovic, Slavica Solujic


Total phenolic content, concentration of flavonoids and in vitro antioxidant activity of twenty different extracts from the whole plant and plant parts (leaves, flowers and stems) of Teucrium polium were determined. The total phenolic contents ranged between 14.57 to 157.84 mg of GaA/g of extract. The concentrations of flavonoids varied from 6.48 to 139.87 mg of Ru/g of extract. Antioxidant activity was determined in vitro using DPPH reagent and expressed as concentration of each extract required to inhibit radical by 50% (IC50) values that ranged from 26.30 to 2190.75 µg/ml. The methanolic leaves extract contain the greatest concentration of phenolic compounds (157.84 mg of GaA/g) and showed strong antioxidant activity (IC50 = 26.30 µg/ml). Ginkgo and Green tea extracts were analyzed for comparison, and the results indicated that some extracts of T. polium were equal in activity with Ginkgo or Green tea and some appeared to have greater activity. The obtained results suggest strong antioxidant activity and large contribution of separate analysis for the maximum exploitation of active phenolic compounds from T. polium. Based on this information, plant parts of this plant are natural sources of antioxidant substances of high importance.


Teucrium polium subsp. polium; antioxidant activity; phenolics; flavonoids

Full Text:



Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem. 2001;49(11):5165-5170.

Halliwell B, Rafter J, Jenner A. Health promotion by flavonoids, tocopherols, tocotrienols, and other phenols: direct or indirect effects? Antioxidant or not? Am J Clin Nutr. 2005;81(1):268S-276S.

Rohma A, Riyanto S, Yuniarti N, Saputra WR, Utami R, Mulatsih W. Antioxidant activity, total phenolic, and total flavaonoid of extracts and fractions of red fruit (Pandanus conoideus Lam). Int Food Res J. 2010;17:97-106.

Moein S, Moein RM. Relationship between antioxidant properties and phenolics in Zhumeria majdae. J Med Plants Res. 2010;4(7):517-521.

Diklić N. Teucrium. In: Josifović M, editor. Flore de la Republique Socialiste de Serbie. Belgrade: Serbian Academy of Sciences and Arts; 1974. p. 349-356.

Jurisić R, Vladimir-Knezević S, Kalodera Z, Grgić J. Determination of selenium in Teucrium species by hydride generation atomic absorption spectrometry. Z Naturforsch C J Biosci. 2003;58(1-2):143-145.

Rajabalian S. Methanolic extract of Teucrium polium L. potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines. Exp Oncol. 2008;30(2):133-138.

Shtukmaster S, Ljubuncic P, Bomzon A. The effect of an aqueous extract of Teucrium polium on glutathione homeostasis in vitro: a possible mechanism of its hepatoprotectant action. Adv Pharmacol Sci. 2010;2010:1-7.

Suboh SM, Bilto YY, Aburjai TA. Protective effects of selected medicinal plants against protein degradation, lipid peroxidation and deformability loss of oxidatively stressed human erythrocytes. Phytother Res. 2004;18(4):280-284.

Ljubuncic P, Azaizeh H, Portnaya I, Cogan U, Said O, Saleh KA, et al. Antioxidant activity and cytotoxicity of eight plants used in traditional Arab medicine in Israel. J Ethnopharmacol. 2005;99(1):43-47.

Siddique AN, Mujeeb M, Najmi AK, Akram M. Evaluation of antioxidant activity, quantitative estimation of phenols and flavonoids in different parts of Aegle marmelos. Afr J Plant Sci. 2010;4(1):1-5.

Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Meth Enzymol. 1999;299:152-178.

Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, et al. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol. 2000;72(1-2):35-42.

Takao T, Kitatani F, Watanabe N, Yagi A, Sakata K. A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biosci Biotechnol Biochem. 1994;58(10):1780-1783.

Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of some Scottish plants for free radical scavenging activity. Phytother Res. 2007;21(7):615-621.

Marinova EM, Yanishlieva NV. Antioxidative activity of extracts from selected species of the family Lamiaceae in sunflower oil. Food Chem. 1997;58(3):245-248.

Zarena AS, Sankar KU. A study of antioxidant properties from Garcinia mangostana L. pericarp extract. Acta Sci Pol Technol Aliment. 2009;8:23-34.

Mohsen SM, Ammar ASM. Total phenolic contents and antioxidant activity of corn tassel extracts. Food Chem. 2009;112(3):595-598.

Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010;15(10):7313-7352.

Liu S, Lin J, Wang C, Chen H, Yang D. Antioxidant properties of various solvent extracts from lychee (Litchi chinenesis Sonn.) flowers. Food Chem. 2009;114(2):577-581.

Horax R, Hettiarachchy N, Chen P. Extraction, quantification, and antioxidant activities of phenolics from pericarp and seeds of bitter melons (Momordica charantia) harvested at three maturity stages (immature, mature, and ripe). J Agric Food Chem. 2010;58(7):4428-4433.

Metivier RP, Francis FJ, Clydesdale FM. Solvent extraction of anthocyanins from wine pomace. J Food Sci. 1980;45(4):1099-1100.

Shi J, Nawaz H, Pohorly J, Mittal G, Kakuda Y, Jiang Y. Extraction of polyphenolics from plant material for functional foods-engineering and technology. Food Rev Int. 2005;21(1):139-166.

Silva MM, Santos MR, Caroço G, Rocha R, Justino G, Mira L. Structure-antioxidant activity relationships of flavonoids: a re-examination. Free Radic Res. 2002;36(11):1219-1227.

Leccese A, Viti R, Bartolini S. The effect of solvent extraction on antioxidant properties of apricot fruit. Cent Eur J Biol. 2011;6(2):199-204.

Özen T, Çöllü Z, Korkmaz H. Antioxidant properties of Urtica pilulifera root, seed, flower, and leaf extract. J Med Food. 2010;13(5):1224-1231.