A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

Jakub Sawicki, Monika Szczecińska

Abstract


RAPDs, ISJs, ISSRs, ITS and katGs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia. Twenty populations were genotyped using ten ISJ primers, 12 pairs of katG primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and katG markers revealed the greatest number of species-specific bands. An analysis of ITS1 and ITS2 regions with restriction enzymes also proved to be a highly effective tool for species identification.

Keywords


Sphagnum; genetic similarity; molecular markers RAPD; ISJ; katG; ITS; ISSR

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References


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