Uptake of fluorescent-labeled BSA into root cells: endocytosis?

A. Kaźmierczak, J. Maszewski

Abstract


Incorporation of rhodamine- and fluorescein-isothiocyanate labeled bovine serum albumin (BSA-TRITC, BSA-FITC) was examined in different root zones of the 3-day-old seedlings in Melandrium noctiflorum, Allium cepa and Zea mays. The uptake of fluorescent-labeled BSA was found: (1) species-specific, (2) cell-type dependent, and (3) cytochalasin B-sensitive. The characteristic punctute distribution of vesicles within the cytoplasm suggests the internalization of labeled proteins by endocytosis.

Keywords


endocytosis; Melandrium noctiflorum L.; Allium cepa L.; Zea mays L.; BSA; fluorescein isothiocyanate (FITC); tetramethylrhodamine isothiocyanate (TRITC); cytochalasin B

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DOI: https://doi.org/10.5586/asbp.1997.037

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Polish Botanical Society