Cytological effect of Tilt 250 EC in the successive phases of the Triticale rust disease process caused by Puccinia recondita f. sp. tritici

Abstract

The disease process caused by rust (Puccinia recondita f. sp. tritici) in Triticale (Triticale-Wittmack cv. Bolero) and the effect of the application of the propikonazole - Tilt 250 EC are described. In plants not protected chemically one could observe the whole pathogen developmental cycle ending with the appearance of uredinia with urediniospores. The highest pathogen susceptibility to chemical agent was observed at the time of inoculation, during incubation and at the beginning of the actual disease. By inhibiting the ergosterol biosynthesis in the fungus cells the preparation (propikonazole) inhibited the development of the intra- and extracellular mycelium. The mycelium degeneration manifested itself by the irregular growth of intercellular hyphae, perforation of septa, homogenization of protoplasts and cell collapse. The thickening of the cell wall, modification of the perihaustorial space and protoplast obliteration were observed in the haustoria.