Sequence and expression of a microspore cDNA clone with homology to a ribosomal protein

Michael P. Turcich, Amana Bokhari-Riza, Douglas A. Hamilton, Joseph P. Mascarenhas

Abstract


A cDNA library was made to RNA from corn anthers containing developing pollen at the uninucleate microspore stage. A randomly selected clone from this library which contained an insert (531 bp) was isolated and sequenced. An open reading frame of 330 bp was located. Computer alignments of the putative amino acid sequence with sequences from GenBank and the SwissProt protein databases indicated homology to L12, an acidic ribosomal protein. RNA blot analysis showed highest levels of this mRNA in mature pollen. The significance of this observation in light of the known biochemistry of ribosome synthesis in developing pollen is discussed.

Keywords


pollen; Zea mays; L7/L12 protein; ribosomes

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DOI: https://doi.org/10.5586/asbp.1996.011

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Publisher
Polish Botanical Society