Effect of ammonium nutrition on the nitrate utilization, nitrate reductase actvity and growth of Spirodela polyrrhiza

Ewa Tatkowska, Józef Buczek

Abstract


The influence of NH4+ ions on nitrate assimilation and growth of sterile Spirodela polyrrhiza cultures was investigated. S. polyrrhiza utilises both the nitrate and the ammonium form of nitrogen, it prefers, however, NH4+. Ammonium ions present in the nitrate medium inhibit the activity of nitrate reductase (NR), but they do not affect enzyme 'induction and only slightly reduce N03- uptake. These results sugest that the inhibitory effect of NH4+ on the NR activity is the main cause of the decrease in N03- assimilation by S. polyrrhiza cultures growing in nitrate-ammonium medium.

Keywords


nitrate reductase; nitrate utilization; Spirodela polyrrhiza

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