Regeneration of plants from leaves of Chrysanthemum morifolium Ram. cv. Bronze Bornholm in in vitro cultures

Aurelia Ślusarkiewicz-Jarzina, Maciej Zenkteler, Barbara Podlewska

Abstract


Plants were obtained from cultured in vitro leaves of Chrysanthemum morifolium Ram. cv. Bronze Bornholm. The leaves were inoculated on Murashige and Skoog medium (MS) supplemented with cytokinins (kinetin - KIN, zeatin - ZEA, 6-benzyloaminopurine - BAP) and auxins (2,4-dichlorophenoxyacetic acid - 2,4-D, α-naphtaleneacetic acid - NAA, 3-indolilacetic acid - IAA, p-fluorophenylalanine - PFA) in various combinations and concentra-tions. The most suitable medium was that one which contained 4 mg/l KIN, 2 mg/l NAA and 50 mg/l PFA.

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DOI: https://doi.org/10.5586/asbp.1982.015

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Polish Botanical Society