In vitro culture of Secale cereale L. explants-callus formation and organ differentiation

Abstract

Explants originating from seeds, embryos, seedlings and maturing plants of rye were cultured on a modified Murashige and Skoog medium (MS) and a Schenk and Hildebrandt medium (SH). Depending on various combinations and concentrations of NAA (α-tnaphthaleneacetic acid), IAA (β-indoleacetic acid),2,4-D (dichlosaphenoxyacetic acid), 2,4,5-T(trichloraphenoxyacetic acid),2,4-D (dichloraphenoxyacetic acid), 2,4,5-T (trichloraphenoxyacetic = 2 iP), ZEA (zeatin), and GA₃ (gibberellic acid) mature embryo, isolated radicles and lateral roots formed callus itissue which was only capable of rhizogenesis. Leaf segments of 3 and 5-day-old seedlings produced callus and roots, however the rachis of a spike of 7 mm in length was capable of forming shoots which later developed into plants.