Studies on antigenic differences in needle proteins of Pinus sylvestris L., P. mugo Turra, P. uliginosa Neumann and P. nigra Arnold

Abstract

By means of serological methods (double immunodiffusion, immunoabsortion and quantitative immunoprecipitation), using three kinds of antisera: antisylvestris, antimugo and antiuliginosa authors performed a comparison of antigenic properties of proteins from needles of P. sylvestris, P. mugo, P. uliginosa and P. nigra. Proteins characteristic for the species P. sylvesiris, and P. uliginosa were found. On the basis of the results obtained it was established, that the most distinct species are P. mugo and P. sylvestris, P. uliginosa is antigenically different from the two taxa but is showing greater similarity to P. mugo than to P. sylvestris. P. nigra proteins are different from proteins P. sylvestris, P. mugo, and P. uliginosa. They show however, some antigenic similarity to P. sylvestris proteins.