Abstract
A significant limitation of callus tissue growth was noted in Nicotiana tabacum L. cultured in vitro under the influence of morphactin IT 3233 applied in 10 - 40 mg/dm3 concentrations. Growth inhibition was associated with an increase in dry mass content and the contribution of protein N to the nitrogen, pool. Tumour tissue of tobacco under the same conditions reacted weaker to morphactin. Growth limitation reached 25 per cent as compared with the control, dry mass and nitrogen compounds content showed only slight variations. It is supposed that the different sensitivity of both types of tissues to morphactin is connected with their different hormonal metabolism.