Changes in the electrophoretic pattern of glucosidases during apple seeds stratification
Abstract
The technique of localization of glucosidases on disc electropherograms, based on reduction of 2,3,5-triphenyltetrazolium chloride by enzymatically liberated sugar is described. The changes in electrophoretic patterns of amygdaline, phloridzin and p-nitrophenyl-β-glucoside hydrolysing glucosidases during apple seed stratification were studied. The changes were correlated with earlier described changes in endogenous apple seed amygdaline and phloridzin contents.
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PDFDOI: https://doi.org/10.5586/asbp.1973.013
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