The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions

D. Tyrawska-Spychałowa, Z. Adamczyk, B. Halicz

Abstract


Plant tissue -4 days old germs of Zea mays and Pisum sativum were subjected to ultrasonic energy using generator with a constant frequency of 800 kHz. RNA was isolated by Mieczyński’s modification of the method described by Georgiev. Preparations of RNA were separated by electrophoresis on the coloured agar-gel. Depending on the degree of ultrasonication changes in three fractions of RNA: A1 - 28 S, A2 - 18 S and A3 - 4 S were observed

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DOI: https://doi.org/10.5586/asbp.1973.010

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Publisher
Polish Botanical Society