Somatic embryogenesis in Lolium multiflorum suspension culture

Margarita Pavlova, Elizabeth Kordyum

Abstract


The embryogenic cell suspension was obtained from immature embryos of Lolium multiflorum through a callus culture. Somatic embryogenesis was induced by addition of 2,4-D, dicamba and picloram in 0,5 mg/l concentrations in MS liquid nutrient medium. It was shown that somatic embryos arised from single cells. In globular embryoids, the meristematic cells are characterized by the presence of phytoferritin inclusions in the leucoplasts.

Keywords


immature embryo; suspension culture; ryegrass; somatic embryo; phytoferritin

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DOI: https://doi.org/10.5586/asbp.1996.007

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Publisher
Polish Botanical Society