Accumulation of ixerin F and activities of some terpenoid bisynthetic enzymes in a cell suspension culture of Lactuca virosa L.

Anna Stojakowska, Janusz Malarz, Robert van der Heijden, Robert Verpoorte

Abstract


A cell suspension culture of Lactuca virosa L. (Asteraceae, tribe Lactuceae) is capable of synthesizing sesquiterpene lactones of which ixerin F is the main compound. The culture was characterized on growth (by dissimilation rates), on ixerin F accumulation (by RP-HPLC) and on some enzyme activities involved in early steps of terpenoid biosynthesis. Acetoacetyl-coenzyme A thiolase (AACT, E.C. 2.3.1.9) and 3S-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGS, E.C. 4.13.5) activities of the cells were assayed spectrophotometrically. HMGS activity increased during the culture period and reached a maximum during the stationary phase (190 pkat/mg protein), while AACT showed relatively high level of activity throughout the growth cycle, with transient decrease at the logarithmic growth phase and the beginning of stationary phase. Ixerin F accumulated inside the cells and the maximum concentration of 0.08% (on dry weight basis) was found in the early stationary phase of the growth cycle of the culture.

Keywords


Lactuca virosa L.; ixerin F; acetoacetyl-coenzyme A thiolase (E.C. 2.3.1.9); 3S-hydroxy-3-methylglutaryl-coenzyme A synthase (E.C. 4.1.3.5); sesquiterpene lactones

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DOI: https://doi.org/10.5586/asbp.1997.023

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Polish Botanical Society