Abstract
The analysis of the sets of free and liberated by hydrolysis phenolic acids in the leaves of, Secamone afzelii (Rhoem.) Schult. was conducted by 2D-TLC and RP-HPLC methods. Sixteen phenolic acids were identified: chlorogenic, gallic, protocatechuic, homoprotacatechuic, caffeic, gentysic, α-resorcylic, p-hydroxyphenylacetic, p-hydroxybenzoic, p-coumaric, o-hydroxyphenylacetic, syryngic, vanillic, synapic, ferulic and salicylic. By means of the RP-HPLC the contents of major phenolic acids was described. It is fairly diversified in cases of different compounds ranging from 0.05 up to 16.89 mg%.
Keywords
Secamone afzelii (Rhoem.) Schult.; Asclepiadaceae; phenolic acids; 2D-TLC and RP-HPLC analysis