Email this article 
Critical factors besides treatment dose and duration need to be controlled in Pb toxicity tests in plant cell suspension cultures
Abstract
The study was designed to determine the proper conditions for suspension culture of Viola tricolor cells in toxicity studies of Pb at different concentrations (0, 200, 500, 1000, 2000 µM) and exposure times (24, 48, 72 h). By forming insoluble salts with ions from the medium, lead (II) nitrate added to the medium decreased the initial 5.7–5.8 pH of the medium, depending on the Pb salt concentration and light intensity.
In alamarBlue assays, we found no dose- or time-dependent effect of Pb on cell viability when we did not adjust pH and did not standardize the illumination conditions to correct the effect of lead-salt-induced turbidity.
When effective illumination was adjusted to correct for turbidity at the highest lead concentration and pH was adjusted to 5.7–5.8, cell viability decreased with the increase of Pb(NO3)2 concentration and with treatment time. These experiments demonstrate that the toxic action of lead on cells in suspension depends strongly on culture conditions, and not only on the metal concentration and duration of treatment.Keywords
Full Text:
PDFReferences
Agbogidi OM, Mariere AE, Ohwo OA. Metal concentration in plant tissues of Jatropha curcas L. grown in crude oil contaminated soil. Journal of Sustainable Forestry. 2013;32:404–411. https://doi.org/10.1080/10549811.2011.599099
Bolan N, Kunhikrishnan A, Thangarajan R, Kumpiene J, Park J, Makino T, et al. Remediation of heavy metal(loid)s contaminated soils; to mobilize or immobilize? J Hazard Mater. 2014;266:141–166. https://doi.org/10.1016/j.jhazmat.2013.12.018
Porrut B, Shahid M, Dumat C, Winterton P, Pinelli E. Lead uptake, toxicity, and detoxification in plants. Rev Environ Contam Toxicol. 2011;213:113–136. https://doi.org/10.1007/978-1-4419-9860-6_4
Hettiarachchi GM, Pierzynski GM. Soil lead bioavailability and in situ remediation of lead-contaminated soils: a review. Environ Prog Sustain Energy. 2004;23(1):78–93. https://doi.org/10.1002/ep.10004
Harter RD. Effect of soil pH on adsorption of lead, copper, zinc, and nickel. Soil Sci Soc Am J. 1983;47:47–51. http://doi.org/10.2136/sssaj1983.03615995004700010009x
Norvell WA. Comparison of chelating agents as extractants for metals in diverse soil materials. Soil Sci Soc Am J. 1984;48:1285–1292. https://doi.org/10.2136/sssaj1984.03615995004800060017x
Bernabé-Antonio A, lvarez LA, Buendýa-González L, Maldonado-Magaňa A, Cruz-Sosa F. Accumulation and tolerance of Cr and Pb using a cell suspension culture system of Jatropha curcas. Plant Cell Tissue Organ Cult. 2015;120:221–228. https://doi.org/10.1007/s11240-014-0597-y
Lizhong Z, Cullen WR. Effects of some heavy metals on cell suspension cultures of Catharanthus roseus. Journal of Environmental Sciences. 1995;7(1):60–65
Maldonado-Magaňa A, Orozco-Villafuerte J, Buendia-González L, Estrada-Zuňiga ME, Bernabé-Antonio A, Cruz-Sosa F. Establishment of cell suspension cultures of Prosopis laevigata (Humb. & Bonpl. ex Willd) M. C. Johnst to determine the effect of zinc on the uptake and accumulation of lead. Revista Mexicana de Ingenieria Quimica. 2013;12(3):489–498
Poborilova Z, Opatrilova R, Babula P. Toxicity of aluminium oxide nanoparticles demonstrated using a BY-2 plant cell suspension culture model. Environ Exp Bot. 2013;91:1–11. https://doi.org/10.1016/j.envexpbot.2013.03.002
Ding M, Wang X, Li Y. Acquired tolerance to cadmium following long-term acclimation to CdCl2 in rice suspension cultures. Plant Cell Tissue Organ Cult. 2016;124:47–55. https://doi.org/10.1007/s11240-015-0873-5
Clever HL, Johnston FJ. The solubility of some sparingly soluble lead salts: an evaluation of the solubility in water and aqueous electrolyte solution. J Phys Chem Ref Data. 1980;9:3. https://doi.org/10.1063/1.555628
Słomka A, Libik-Konieczny M, Kuta E, Miszalski Z. Metallifierous and non-metalliferous populations of Viola tricolor represent similar mode of antioxidative response. J Plant Physiol. 2008;165:1610–1619. https://doi.org/10.1016/j.jplph.2007.11.004
Słomka A, Kuta E, Szarek-Łukaszewska G, Godzik B, Kapusta P, Tylko G, et al. Violets of the section Melanium, their colonization by arbuscular mycorrhizal fungi and their occurrence on heavy metal heaps. J Plant Physiol. 2011;168:1191–1199. https://doi.org/10.1016/j.jplph.2011.01.033
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 1962;15:473–479. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Zhang YK, Zhu DF, Zhang YP, Chen HZ, Xiang J, Lin XQ. Low pH-induced changes of antioxidant enzyme and ATPase activities in the roots of rice (Oryza sativa L.) seedlings. PLoS One. 2015;10(2):e0116971. https://doi.org/10.1371/journal.pone.0116971
Soares EV, Duarte APRS, Soares HMVM. Study of the suitability of 2-(N-morpholino) ethanesulfonic acid pH buffer for heavy metals accumulation studies using Saccharomyces cerevisiae. Chemical Speciation and Bioavailability. 2000;12(2):59–65. https://doi.org/10.3184/095422900782775535
Salonikidis PS, Kirichenko SN, Tatjanenko LV, Schwarz W, Vasilets LA. Extracellular pH modulates kinetics of the Na+, K+-ATPase. Biochim Biophys Acta. 2000;1509:496–504. https://doi.org/10.1016/S0005-2736(00)00356-4
DOI: https://doi.org/10.5586/asbp.3555
|
|
|
