Abstract
Direct addition of pectinase as an elicitor to the hairy root culture of Lactuca virosa L. transformed with Agrobacterium rhizogenes stimulates the accumulation of constitutive sesquiterpene lactones - lactuside A and crepidiaside B. The highest amounts of lactuside A (80% above the control level) and crepidiaside B (39% above the control level) have been found 24 and 48 hours, respectively, after pectinase treatment.
Keywords
Lactuca virosa L.; Asteraceae; hairy root culture; Agrobacterium rhizogenes; pectinase; elicitor; lactuside A; crepidiaside B; sesquiterpene lactones