Wounding stimulates ALLENE OXIDE SYNTHASE gene and increases the level of jasmonic acid in Ipomoea nil cotyledons

Emilia Wilmowicz, Agata Kućko, Kamil Frankowski, Barbara Zabrocka-Nowakowska, Katarzyna Panek, Jan Kopcewicz

Abstract


Allene oxide synthase (AOS) encodes the first enzyme in the lipoxygenase pathway, which is responsible for jasmonic acid (JA) formation. In this study we report the molecular cloning and characterization of InAOS from Ipomoea nil. The full-length gene is composed of 1662 bp and encodes for 519 amino acids. The predicted InAOS contains PLN02648 motif, which is evolutionarily conserved and characteristic for functional enzymatic proteins. We have shown that wounding led to a strong stimulation of the examined gene activity in cotyledons and an increase in JA level, which suggest that this compound may be a modulator of stress responses in I. nil.

Keywords


allene oxide synthase; gene expression; jasmonates; phytohormones; wounding; Ipomoea nil

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References


Schaller A, Stintzi A. Enzymes in jasmonate biosynthesis – structure, function, regulation. Phytochem. 2009;70(13–14):1532–1538. http://dx.doi.org/10.1016/j.phytochem.2009.07.032

Acosta IF, Farmer EE. Jasmonates. The Arabidopsis Book. 2010;8:e0129. http://dx.doi.org/10.1199/tab.0129

von Malek B, van der Graaff E, Schneitz K, Keller B. The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway. Planta. 2002;216(1):187–192. http://dx.doi.org/10.1007/s00425-002-0906-2

Howe GA, Lee GI, Itoh A, Li L, DeRocher AE. Cytochrome P450-dependent metabolism of oxylipins in tomato. Cloning and expression of allene oxide synthase and fatty acid hydroperoxide lyase. Plant Physiol. 2000;123(2):711–724. http://dx.doi.org/10.1104/pp.123.2.711

Maucher H, Hause B, Feussner I, Ziegler J, Wasternack C. Allene oxide synthases of barley (Hordeum vulgare cv. Salome): tissue specific regulation in seedling development. Plant J. 2000;21(2):199–213. http://dx.doi.org/10.1046/j.1365-313x.2000.00669.x

Froehlich JE, Itoh A, Howe GA. Tomato allene oxide synthase and fatty acid hydroperoxide lyase, two cytochrome P450s involved in oxylipin metabolism, are targeted to different membranes of chloroplast envelope. Plant Physiol. 2001;125(1):306–317. http://dx.doi.org/10.1104/pp.125.1.306

Wilmowicz E, Frankowski K, Glazińska P, Kęsy J, Wojciechowski W, Kopcewicz J. Cross talk between phytohormones in the regulation of flower induction in Pharbitis nil. Biol Plant. 2011;55(4):757–760. http://dx.doi.org/10.1007/s10535-011-0182-8

Frankowski K, Wilmowicz E, Kućko A, Kopcewicz J. Molecular cloning of BLADE-ON-PETIOLE gene and expression analyses during nodule development in Lupinus luteus. J Plant Physiol. 2014;179:35–39. http://dx.doi.org/10.1016/j.jplph.2015.01.019

Wilmowicz E, Frankowski K, Grzegorzewska W, Kęsy J, Kućko A, Banach M, et al. The role of jasmonates in the formation of a compound of chalcones and flavans with phytoalexin-like properties in mechanically wounded scales of Hippeastrum × hybr. bulbs. Acta Biol Crac Ser Bot. 2014;56(1):54–58. http://dx.doi.org/10.2478/abcsb-2014-0007

Itoh A, Schilmiller AL, McCaig BC, Howe GA. Identification of a jasmonate-regulated allene oxide synthase that metabolizes 9-hydroperoxides of linoleic and linolenic acids. J Biol Chem. 2002;277:46051–46058. http://dx.doi.org/10.1074/jbc.M207234200

Laudert D, Weiler EW. Allene oxide synthase: a major control point in Arabidopsis thaliana octadecanoid signaling. Plant J. 1998;15:675–684. http://dx.doi.org/10.1046/j.1365-313x.1998.00245.x

Glauser G, Grata E, Dubugnon L, Rudaz S, Farmer EE, Wolfender JL. Spatial and temporal dynamics of jasmonate synthesis and accumulation in Arabidopsis in response to wounding. J Biol Chem. 2008;283:16400–16407. http://dx.doi.org/10.1074/jbc.M801760200

Gullner G, Künstler A, Király L, Pogány M, Tobia I. Up-regulated expression of lipoxygenase and divinyl ether synthase genes in pepper leaves inoculated with Tobamoviruses. Physiol Mol Plant Pathol. 2010;74:387–393. http://dx.doi.org/10.1016/j.pmpp.2010.06.006