Velocity of cytoplasm streaming in basal and subbasal cells of antheridium as well as internodal cells of pleuridium in Chara vulgaris L. and GA3 influence on it: videomicroscopic observations

Abstract

The velocity of cytoplasm streaming in an antheridial basal cell and in a subbasal cell as well as in internodal cells of pleuridia carrying antheridia were measured with the use of videomicroscopy. Velocity of streaming proved different depending on a cell type. The most intensive streaming (ca 40 µm/s) was observed in a subbasal cell while in a basal cell it was quite intensive during antheridial filament cells proliferation but falling to half of it during spermatozoid differentiation (ca 20 µm/s and 10 µm/s respectively). In internodal cells of pleuridia the velocity was ca 17 µm/s. GA₃ at the 10^-5M concentration decreased the velocity of streaming in a basal cell during proliferation of antheridial filament cells and increased it during spermiogenesis. In internodal cells of pleuridia the velocity diminished while in a subbasal cell it rose a little after GA₃ administering. The obtained data suggest that cytoplasm streaming and its reaction to exogenous gibberellin depend on the role of a cell in a multicellulate system; it also depends on a developmental stage.