Studies on pigments of the myxomycete Physarum nudum. II. Separation and optical properties of the pigments from plasmodia cultured in darkness

L. Raczkowski


The paper presents the data on Separation and some optical properties of the pigments obtained from the plasmodium of the myxomycete Physarum nudum grown in the dark. Pigment Separation was performed by means of thin-layer chromatography with celulose MN 300 as adsorbent and with the solvent: tert.-butylalcohol, H2O, 3N NH4OH at the ratio 5:2:1 In these conditions the chromatograms revealed 12 coloured bands from which pigments were eluted and their absorption spectra as well as the spectra of fluorescence emission were determined. The isolated pigments differ from one another by their physical properties (different Rf values, localization of absorption maxima, and behaviour in acid solution). Nevertheless, certain analogies perceptible between particular pigments per-mitted to distinguish 3 families of the studied pigments demonstrating similar properties.

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