Changes in the electrophoretic pattern of glucosidases during apple seeds stratification

A. Podstolski, St. Lewak

Abstract


The technique of localization of glucosidases on disc electropherograms, based on reduction of 2,3,5-triphenyltetrazolium chloride by enzymatically liberated sugar is described. The changes in electrophoretic patterns of amygdaline, phloridzin and p-nitrophenyl-β-glucoside hydrolysing glucosidases during apple seed stratification were studied. The changes were correlated with earlier described changes in endogenous apple seed amygdaline and phloridzin contents.

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DOI: https://doi.org/10.5586/asbp.1973.013

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Publisher
Polish Botanical Society