Detection of potato virus X in potato leaves by means of polyacrylamide gel electrophoresis

Marta Czupryn, Maria Błaszczyk

Abstract


A rapid method of detection and evaluation of potato virus X (PVX) infection in potato leaves is described. The method is based on: 1) discarding of major part of cytoplasmic proteins from leaf homogenate by precipitation with polyethylene glycol in final concentration of 2%, 2) precipitation of virus containing fraction by increasing the concentration of polyethylene glycol to 3.5% and electrophoretic identification of virus coat protein extracted from this fraction. The procedure allows early detection of PVX in plants inoculated with virus as well as grown from infected tubers.

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DOI: https://doi.org/10.5586/asbp.1974.049

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Publisher
Polish Botanical Society