Metabolic aspects of growth in HU-treated crown-gall tissue cultures. II. Helianthus annuus

Aldona Rennert


The dynamics of growth and changes in nucleic acid and protein contents in sunflower calluses and tumours cultured in hydroxyurea (HU) containing media were examined. HU-induced changes in healthy tissues ran in parallel always in the same direction, in tumourous ones however an uncoupling between DNA synthesis and tissue growth on one hand and RNA and protein synthesis on the other took place. A detailed analysis of the results allows to suppose that the specific activity of HU on tumourous tissue could be an index of: 1) quantitative disturbances in its genes function (2) degree of the lass of sensitivity to the factors of regulation.

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