Isolation of 'mating fraction' from Chlamydomonas reinhardtii gametic flagellum membranes by affinity chromatography

Aleksander F. Sikorski, Wanda Mejbaum-Katzenellenbogen

Abstract


Solubilized with Triton X-100 strain 137+ gametic flagellum membrane material was bound to CNBr-activated Sepharose 4B, and used for affinity chromatography of labeled with 125I 89 gametic flagellum membrane soluble in Triton X-100. A single peak containing 2.7-4.7% of total radioactivity was obtained upon pH change of the eluting buffer. Upon re-chromatography 50-70% of this material was adsorbed and eluted. The complex was found to be stable at pH 7.0-8.0 in the presence of divalent cations (Ca+2 and Mg+2).

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DOI: https://doi.org/10.5586/asbp.1979.044

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Polish Botanical Society