Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins

Aleksander F. Sikorski

Abstract


SDS-polyacrylamide electrophoretic patterns of Chlamydomonas flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and internal localization of fractions '4' and '6'. Application of SDS-polyacrylamide gel electrophoresis and radiolabeled membranes allowed to isolate individual membrane polypeptides.

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DOI: https://doi.org/10.5586/asbp.1979.042

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Publisher
Polish Botanical Society