Effects of selenium on the growth and photosynthetic characteristics of flue-cured tobacco (Nicotiana tabacum L.)

Chaoqiang Jiang, Chaolong Zu, Jia Shen, Fuwen Shao, Tian Li

Abstract


The objective of this study was to investigate the effect of Selenium (Se) supply (0, 3, 6, 12, 24 mg kg−1) on the growth, photosynthetic characteristics, Se accumulation and distribution of flue-cured tobacco (Nicotiana tabacum L.). Results showed that low-dose Se treatments (≤6 mg kg−1) stimulated plant growth but high-dose Se treatments (≥12 mg kg−1) hindered plant growth. Optimal Se dose (6 mg kg−1) stimulated plant growth by reducing MDA content and improving photosynthetic capability. However, excess Se (24 mg kg−1) increased MDA content by 28%, decreased net photosynthetic rate and carboxylation efficiency by 34% and 39%, respectively. The Se concentration in the roots, stems, and leaves of the tobacco plants significantly increased with increasing Se application. A linear correlation (R = 0.95, P < 0.01) was observed between Se level and tobacco plant tissue Se concentration. This correlation indicated that the tobacco plant tissues were not saturated within the concentration range tested. The pattern of total Se concentration in the tobacco plant tissues followed the order root > leaf > stem. The Se concentration in the roots was 3.17 and 7.57 times higher than that in the leaves and stems, respectively, after treatment with 24 mg kg−1 Se. In conclusion, the present study suggested that optimal Se dose (6 mg kg−1) improved the plant growth mainly by enhancing photosynthesis, stomatal conductance, carboxylation efficiency and Rubisco content in the flue-cured tobacco leaves. However, the inhibition of excess Se on tobacco growth might be due to high accumulation of Se in roots and the damage of photosynthesis in leaves.

Keywords


flue-cured tobacco; selenium (Se); photosynthesis; carboxylation efficiency; Rubisco content

Full Text:

PDF

References


Terry N, Zayed AM, de Souza MP, Tarun AS. Selenium in higher plants. Annu Rev Plant Physiol Plant Mol Biol. 2000;51(1):401–432. http://dx.doi.org/10.1146/annurev.arplant.51.1.401

Feng R, Wei C, Tu S. The roles of selenium in protecting plants against abiotic stresses. Environ Exp Bot. 2013;87:58–68. http://dx.doi.org/10.1016/j.envexpbot.2012.09.002

Hartikainen H, Xue T, Piironen V. Selenium as an anti-oxidant and pro-oxidant in ryegrass. Plant Soil. 2000;225(1–2):193–200. http://dx.doi.org/10.1023/A:1026512921026

van Hoewyk D. A tale of two toxicities: malformed selenoproteins and oxidative stress both contribute to selenium stress in plants. Ann Bot. 2013;112(6):965–972. http://dx.doi.org/10.1093/aob/mct163

Lyons GH, Stangoulis JCR, Graham RD. Tolerance of wheat (Triticum aestivum L.) to high soil and solution selenium levels. Plant Soil. 2005;270(1):179–188. http://dx.doi.org/10.1007/s11104-004-1390-1

Zhu YG, Pilon-Smits EAH, Zhao FJ, Williams PN, Meharg AA. Selenium in higher plants: understanding mechanisms for biofortification and phytoremediation. Trends Plant Sci. 2009;14(8):436–442. http://dx.doi.org/10.1016/j.tplants.2009.06.006

Martin AL, Trelease SF. Absorption of selenium by tobacco and soy beans in sand cultures. Am J Bot. 1938;5(7):380–385. http://dx.doi.org/10.2307/2436764

Turakainen M, Hartikainen H, Seppänen MM. Effects of selenium treatments on potato (Solanum tuberosum L.) growth and concentrations of soluble sugars and starch. J Agr Food Chem. 2004;52(17):5378–5382. http://dx.doi.org/10.1021/jf040077x

Hu Q, Xu J, Pang G. Effect of selenium on the yield and quality of green tea leaves harvested in early spring. J Agr Food Chem. 2003;51(11):3379–3381. http://dx.doi.org/10.1021/jf0341417

Liu Q, Wang DJ, Jiang XJ, Cao ZH. Effects of the interactions between selenium and phosphorus on the growth and selenium accumulation in rice (Oryza sativa). Environ Geochem Health. 2004;26(2):325–330. http://dx.doi.org/10.1023/B:EGAH.0000039597.75201.57

Djanaguiraman M, Prasad PVV, Seppänen M. Selenium protects sorghum leaves from oxidative damage under high temperature stress by enhancing antioxidant defense system. Plant Physiol Bioch. 2010;48(12):999–1007. http://dx.doi.org/10.1016/j.plaphy.2010.09.009

Xu J, Zhu S, Yang F, Cheng L, Hu Y, Pan G, et al. The influence of selenium on the antioxidant activity of green tea. J Sci Food Agric. 2003;83(5):451–455. http://dx.doi.org/10.1002/jsfa.1405

Carey AM, Scheckel KG, Lombi E, Newville M, Choi Y, Norton G, et al. Grain accumulation of selenium species in rice (Oryza sativa L.). Environ Sci Technol. 2012;46 (10):5557–5564. http://dx.doi.org/10.1021/es203871j

Pyrzynska K. Selenium speciation in enriched vegetables. Food Chem. 2009;114:1183–1191. http://dx.doi.org/10.1016/j.foodchem.2008.11.026

Finley JW. Reduction of cancer risk by consumption of selenium-enriched plants: enrichment of broccoli with selenium increases the anticarcinogenic properties of broccoli. J Med Food. 2003;6(1):19–26. http://dx.doi.org/10.1089/109662003765184714

Lu XP, Gui YJ, Xiao BG, Li YP, Tong ZJ, Liu Y, et al. Development of DArT markers for a linkage map of flue-cured tobacco. Chin Sci Bull. 2013;58(6):641–648. http://dx.doi.org/10.1007/s11434-012-5453-z

Jiang C, Zheng Q, Liu Z, Liu L, Zhao G, Long X, et al. Seawater-irrigation effects on growth, ion concentration, and photosynthesis of transgenic poplar overexpressing the Na+/H+ antiporter AtNHX1. J Plant Nutr Soil Sci. 2011;174(2):301–310. http://dx.doi.org/10.1002/jpln.201000033

Jiang C, Zheng Q, Liu Z, Xu W, Liu L, Zhao G, et al. Overexpression of Arabidopsis thaliana Na+/H+ antiporter gene enhanced salt resistance in transgenic poplar (Populus × euramericana ‘Neva’). Trees. 2012;26(3):685–694. http://dx.doi.org/10.1007/s00468-011-0635-x

Li Y, Yang XX, Ren BB, Shen QR, Guo SW. Why nitrogen use efficiency decreases under high nitrogen supply in rice (Oryza sativa L.) seedlings. J Plant Growth Regul. 2012;31:47–52. http://dx.doi.org/10.1007/s00344-011-9218-8

Gao J, Liu Y, Huang Y, Lin ZQ, Bañuelos GS, Lam MHW, et al. Daily selenium intake in a moderate selenium deficiency area of Suzhou, China. Food Chem. 2011;126(3):1088–1093. http://dx.doi.org/10.1016/j.foodchem.2010.11.137

Zhou XB, Shi WM, Zhang LH. Iron plaque outside roots affects selenite uptake by rice seedlings (Oryza sativa L.) grown in solution culture. Plant Soil. 2007;290(1–2):17–28. http://dx.doi.org/10.1007/s11104-006-9072-9

Yao X, Chu J, Wang G. Effects of selenium on wheat seedlings under drought stress. Biol Trace Elem Res. 2009;130(3):283–290. http://dx.doi.org/10.1007/s12011-009-8328-7

Wang YD, Wang X, Wong YS. Proteomics analysis reveals multiple regulatory mechanisms in response to selenium in rice. J Proteomics. 2012;75(6):1849–1866. http://dx.doi.org/10.1016/j.jprot.2011.12.030

Chen TF, Zheng WJ, Luo Y, Yang F, Bai Y, Tu F. Effects of selenium stress on photosynthetic pigment contents and growth of Chlorella vulgaris. J Plant Physiol Mol Biol. 2005;31(4):369–373. http://dx.doi.org/10.3321/j.issn:1671-3877.2005.04.006

Azevedo Neto AD, Prico JT, Enéas-Filho J, Braga de Abreu CE, Gomes-Filho E. Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environ Exp Bot. 2006;56(1):87–94. http://dx.doi.org/10.1016/j.envexpbot.2005.01.008

Cartes P, Gianfreda L, Mora ML. Uptake of selenium and its antioxidant activity in ryegrass when applied as selenate and selenite forms. Plant Soil. 2005;276 (1–2):359–367. http://dx.doi.org/10.1007/s11104-005-5691-9

Cartes P, Gianfreda L, Paredes C, Mora ML. Selenium uptake and its antioxidant role in ryegrass cultivars as affected by selenite seed pelletization. J Soil Sci Plant Nut. 2011;11(4):1–14. http://dx.doi.org/10.4067/S0718-95162011000400001

White PJ, Bowen HC, Marshall B, Broadley MR. Extraordinarily high leaf selenium to sulfur ratios define “Se-accumulator” plants. Ann Bot. 2007;100(1):111–118. http://dx.doi.org/10.1093/aob/mcm084

Galeas ML, Zhang LH, Freeman JL, Wegner M, Pilon-Smits EAH. Seasonal fluctuations of selenium and sulfur accumulation in selenium hyperaccumulators and related nonaccumulators. New Phytol. 2007;173:517–525. http://dx.doi.org/10.1111/j.1469-8137.2006.01943.x

Valdez Barillas JR, Quinn CF, Freeman JL, Lindblom SD, Fakra SC, Mar-cus MA, et al. Selenium distribution and speciation in the hyperaccumulator Astragalus bisulcatus and associated ecological partners. Plant Physiol. 2012;159(4):1834–1844. http://dx.doi.org/10.1104/pp.112.199307

Hasanuzzaman M, Hossain MA, Fujita M. Selenium in higher plants: physiological role, antioxidant metabolism and abiotic stress tolerance. J Plant Sci. 2010;5:354–375. http://dx.doi.org/10.3923/jps.2010.354.375

El Mehdawi AF, Pilon-Smits EAH. Ecological aspects of plant selenium hyperaccumulation. Plant Biol. 2012;14:1–10. http://dx.doi.org/10.1111/j.1438-8677.2011.00535.x




DOI: https://doi.org/10.5586/asbp.2015.006

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
This is an Open Access journal, which distributes its content under the terms of the Creative Commons Attribution License, which permits redistribution, commercial and non-commercial, provided that the content is properly cited.
The journal is a member of the Committee on Publication Ethics (COPE) and aims to follow the COPE’s principles.
The journal publisher is a member of the Open Access Scholarly Publishers Association.
The journal content is indexed in Similarity Check, the Crossref initiative to prevent scholarly and professional plagiarism.
Publisher
Polish Botanical Society