Chlorophyll content and culture of flax embryos in vitro

A. Petrova, M. Ciamporova, O. Erdelska

Abstract


During in vitro cultivation of green flax embryos excised 14 days after fertilization on White's medium the chloraphylls a and b are gradually destroyed and the lamellar system of plastids is disintegrated. It was possible, to prolong somewhat the presence of chlorophyll and to enhance embryo growth by culture in the dark and by adding kinetin. Light has a stimulating effect on the germination of embryos. Streptomycin inhibits chlorophyll synthesis not earlier than at germination. Young, green embryos are unable to sumvive and develop on media without sucrose. It is supposed that photosynthesis does not occur in flax embryos in vitro, and that green embryos are not more autotrophic in vitro than those of leucoembryophytes.

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DOI: https://doi.org/10.5586/asbp.1981.047

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Publisher
Polish Botanical Society