Biochemical activity of di- and polyamines in the green alga Chlorella vulgaris Beijerinck (Chlorophyceae)

Romuald Czerpak, Andrzej Bajguz, Alicja Piotrowska, Renata Dobrogowska, Marzena Matejczyk, Wojciech Wiesławski

Abstract


This study concerns on the influence of diamines (agmatine, putrescine) and polyamines (spermine, spermidine) upon the growth and the content of chlorophyll a and b, monosaccharides and proteins in the cells of alga Chlorella vulgaris Beijerinck (Chlorophyceae). In the experiments agmatine, putrescine, spermine and spermidine in the range of concentrations 10-6-10-3 M were used. At the concentration 10-3 M and the 1st day of cultivation, they have a toxic effect on growth of the algae. It was found that di- and polyamines used within the range of concentration 10-6-10-4 M stimulate the growth and the contents of analysed biochemical parameters in the cells of C. vulgaris. The most stimulating influence on metabolism of the alga was demonstrated by spermidine and putrescine at concentration of 10-4 M. Agmatine and spermine were characterised by a lower biological activity than spermidine and putrescine demonstrated the most stimulating influence.

Keywords


Chlorella vulgaris; di- and polyamines; growth; chlorophylls; monosaccharides; proteins

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