Effect of fluorophenylalanine on indole-3-acetic acid levels in Aveaa coleoptiles

Andrew B. Maksymowych, Joseph A. Orkwiszewski

Abstract


The effect of the amino acid analogue D,L-p-fluorophenylalanine on indole-3-acetic acid levels in Avena has been examined. Previous studies have established that D,L-p-fluorophenylalanine promotes elongation, lowers phenolic levels and depresses auxin oxidase activity of etiolated Avena coleoptiles. This study employs an enzyme immunoassay to measure endogenous indole-3-acetic acid concentrations in coleoptile apices. These data demonstrate that treatment of Avena coleoptiles with D,L-p-fluorophenylalanine results in altered auxin levels and help clarify the mechanism of D,L-p-fluorophenylalanine action in Avena

Keywords


Avena coleoptile; enzyme immunoassay; fluorophenylalanine; indole-3-acetic acid; phenolic metabolism

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DOI: https://doi.org/10.5586/asbp.1988.048

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Publisher
Polish Botanical Society