Phenolic acids in extracts obtained from the flowering herbs of Cirsium vulgare (Savi) Ten. growing in Poland

Małgorzata Kozyra, Kazimierz Głowniak

Abstract


In this work the phenolic acids in the methanol extract from the flowering herbs of Cirsium vulgare (Savi) Ten. growing in Poland were isolated and identified. The samples containing free phenolic acids and those released after acid and alkaline hydrolyses were investigated by 2D TLC on cellulose. After purification by SPE, samples were also analyzed by RP-HPLC. Six phenolic acids such as gallic, protokatechuic, gentisic, hydrobenzoic, vanillic and caffeic acids were detected in the fraction of free phenolic acids of the methanol extract, irrespectively of the method used.

Keywords


Cirsium vulgare (Savi) Ten.; phenolic acids

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References


Nazaruk J, Brzóska T. Aktualny stan wiedzy na temat aktywności farmakologicznej roślin z rodzaju Cirsium Mill. Post Fitoter. 2008;3:170–175.

Borawska MH, Czechowska SK, Markiewicz R, Socha K, Nazaruk J, Pałka J, et al. Enhancement of antibacterial effects of extracts from Cirsium species using sodium picolinate and estimation of their toxicity. Nat Prod Res. 2010;24(6):554–561. http://dx.doi.org/10.1080/14786410902770728

Nazaruk J, Czechowska SK, Markiewicz R, Borawska MH. Polyphenolic compounds and in vitro antimicrobial and antioxidant activity of aqueous extracts from leaves of some Cirsium species. Nat Prod Res. 2008;22(18):1583–1588. http://dx.doi.org/10.1080/14786410701825053

Nazaruk J, Jakoniuk P. Flavonoid composition and antimicrobial activity of Cirsium rivulare (Jacq.) All. flowers. J Ethnopharmacol. 2005;102(2):208–212. http://dx.doi.org/10.1016/j.jep.2005.06.012

Yin J, Heo SI, Wang MH. Antioxidant and antidiabetic activities of extracts from Cirsium japonicum roots. Nutr Res Pr. 2008;2(4):247. http://dx.doi.org/10.4162/nrp.2008.2.4.247

Fernández-Martínez E, Díaz-Espinoza R, Villavicencio-Nieto MA, Pérez-Escandón BE, Pérez-Hernández N, Macías A, et al. Preliminary phytochemical and biological study of Cirsium ehrenbergii. Proc West Pharmacol Soc. 2007;50:162–164.

Harborne JB. Flavonoid profiles in the Compositae. In: Heywood VH, Harborne JB, Turner BL, editors. The biology and chemistry of the Compositae. London: Academic Press; 1997. p. 359–384.

Iwashina T, Kadota Y, Ueno T, Ootani S. Foliar flavonoid composition in Japanese Cirsium species (Compositae), and their chemotaxonomic significance. J Jap Bot. 1995;70:280–290.

Jordon-Thaden IE, Louda SM. Chemistry of Cirsium and Carduus: a role in ecological risk assessment for biological control of weeds? Biochem Syst Ecol. 2003;31(12):1353–1396. http://dx.doi.org/10.1016/S0305-1978(03)00130-3

Mabry TJ, Markham KR, Thomas MB. The systematic identification of flavonoids. Berlin: Springer; 1970. http://dx.doi.org/10.1007/978-3-642-88458-0

Moerman DE. Native American ethnobotany. Portland OR: Timber Press; 1998.

Nazaruk J. Antioxidant activity and total phenolic content in Cirsium five species from north-east region of Poland. Fitoterapia. 2008;79(3):194–196. http://dx.doi.org/10.1016/j.fitote.2007.11.008

Yin J, Heo SI, Wang MH. Antioxidant and antidiabetic activities of extracts from Cirsium japonicum roots. Nutr Res Pr. 2008;2(4):247. http://dx.doi.org/10.4162/nrp.2008.2.4.247

Ibrahim RK, Towers GHN. The identification, by chromatography, of plant phenolic acids. Arch Biochem Biophys. 1960;87(1):125–128. http://dx.doi.org/10.1016/0003-9861(60)90132-6

Schmidtlein H, Herrmann K. Quantitative analysis for phenolic acids by thin-layer chromatography. J Chromatogr A. 1975;115(1):123–128. http://dx.doi.org/10.1016/S0021-9673(00)89024-X

Smolarz HD, Waksmundzka–Hajnos M. Two dimensional TLC of phenolic acids on cellulose. J Planar Chromatogr. 1993;6:278–281.

Glensk M, Sawicka U, Mażol I, Cisowski W. 2D TLC-Graft planar chromatography in the analysis of a mixture of phenolic acids. J Planar Chromatogr. 2002;15(6):463–465. http://dx.doi.org/10.1556/JPC.15.2002.6.14

Stalikas CD. Extraction, separation, and detection methods for phenolic acids and flavonoids. J Sep Sci. 2007;30(18):3268–3295. http://dx.doi.org/10.1002/jssc.200700261

Gudej J, Tomczyk M. Chromatographic investigation of polyphenolic compounds from herb Borago officinalis (L.). Herba Pol. 1996;42:252–256.

Hasan A, Waterman P, Iftikhar N. The use of new chromatographic techniques for the isolation and purification of phenolic acids from Indigofera heterantha. J Chromatogr A. 1989;466:399–402. http://dx.doi.org/10.1016/S0021-9673(01)84637-9

Snook ME, Fortson PJ, Chortyk OT. Gel chromatography for the isolation of phenolic acids from tobacco leaf. Anal Chem. 1981;53(2):374–377. http://dx.doi.org/10.1021/ac00225a061

Turek S, Cisowski W. Free and chemically bonded phenolic acids in barks of Viburnum opulus L. and Sambucus nigra L. Acta Pol Pharm. 2007;64(4):377–383.

Nazaruk J, Szoka Ł. The qualitative and quantitative analysis of phenolic acids and flavonoids in Cirsium spp. Herba Pol. 2009;55(4):32–37.

Zgórka G, Hajnos A. The application of solid-phase extraction and reversed phase high-performance liquid chromatography for simultaneous isolation and determination of plant flavonoids and phenolic acids. Chromatographia. 2003;57(1):77–80. http://dx.doi.org/10.1007/BF02492087




DOI: https://doi.org/10.5586/asbp.2013.039

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