The vanadate-sensitive ATPase from plasma membrane of maize roots

Tatiana Pšenáková, Slavomira Kašiarová

Abstract


Plasma membrane vesicles isolated from corn roots were shown to possess Mg2+- dependent and K+ - stimulated ATPase activity, which was inhibited by vanadate, insensitive to nitrate and molybdate, and very little sensitive to azide. The optimum of phosphohydrolase activity was at pH 6.5 and temperature optimum 40oC. ATP was the preferred substrate. The Plasma membrane ATPase was reconstituted into soybean phospholipid liposomes.

Keywords


H+-ATPase, corn root, plasma membrane, reconstitution

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DOI: https://doi.org/10.5586/asbp.1993.026

Journal ISSN:
  • 2083-9480 (online)
  • 0001-6977 (print; ceased since 2016)
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Publisher
Polish Botanical Society