Immunological comparison of the NADH:nitrate reductase from different cucumber tissues

Jolanta Marciniak, Grażyna Kłobus, Józef Buczek, Tadeusz Stefaniak, Jarosław Mazur


Soluble nitrate reductase from cucumber roots (Cucumis sativus L.) was isolated and purified with blue-Sepharose 4B. Specific antibodies against the NR protein were raised by immunization of a goat. Using polyclonal antibodies anti-NR properties of the nitrate reductase from various cucumber tissues were examined. Experiments showed difference in immuno-logical properties of nitrate reductase (NR) from cotyledon roots and leaves.


nitrate reductase; antibody; dot-blot; immunological assays; Cucumis sativus

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