A total of 561 records of 233 species of fungi are reported from the Polish part of Białowieża Forest as a result of a short-term inventory that was conducted during the 18th Congress of European Mycologists (September 18–29, 2019). Four species new to Poland (Bryocentria brongniartii, Tremella coppinsii, T. hypocenomycis, and Zevadia peroccidentalis), and eight species new to Białowieża Primeval Forest (Hypomyces chrysostomus, Hypomyces rosellus, Lachnellula resinaria, Peniophora lycii, Phellinus viticola, Phlebia subochracea, Pronectria anisospora, and Typhula quisquiliaris) were recorded.
Keywordsfungal diversitylichen diversitynew speciesendangered speciesshort-term inventoryrapid inventoryBiałowieża ForestThis study was funded by 18th Congress of European Mycologists and Polish Mycological Society.Introduction
Białowieża Primeval Forest, covering approximately 1,500 km2, is one of the best preserved old-growth mixed forests in Europe. It is located on the Poland–Belarus border in the North Podlasie Plain (Solon et al., 2018). The preservation of Białowieża Forest was possible because from the fifteenth to the eighteenth century, it was used as a royal hunting reserve. Despite various extent of human use through the ages, large parts of the forest remained relatively free of human impact until the twentieth century (Jaroszewicz et al., 2019; Samojlik et al., 2013; Szwagrzyk, 2016). Modern nature conservation started in 1921 when a “Reserve” forestry was created in Białowieża, encompassing 45.9 km2 of old-growth forest. In 1932, it was transformed into The National Park in Białowieża and in 1947, after World War II, it was restored as Białowieża National Park (Okołów et al., 2009). Białowieża Primeval Forest has been a UNESCO World Heritage Site since 1979 (initially only Polish and Belarusian national parks, the whole Białowieża Forest since 2014). The Polish part of Białowieża Primeval Forest is additionally an EU Natura 2000 site, which includes an overlapping Special Protection Area and Special Area of Conservation. The main purpose of most of these designated sites is to protect the natural processes in the remaining part of the primeval forest from direct human interference (Bobiec, 2002).
In the Polish part of Białowieża Primeval Forest, a lime-oak-hornbeam forest Tilio-Carpinetum is the dominant type of potential natural vegetation. However, only approximately half of the area is currently covered by mixed deciduous forests. The remaining areas were changed to Norway spruce or Scots pine plantations as a result of twentieth century forest management. Nonetheless, many well-preserved, old growth deciduous stands, alder carrs Carici elongatae-Alnetum, pine bogs Vaccinio uliginosi-Pinetum, and other, less common forest types, are protected within the nature reserves (Jaroszewicz et al., 2019; Sokołowski, 2004). In numerous areas, a natural mosaic of habitats and vegetation types have been preserved. Moreover, forest microhabitats such as deadwood, animal carcasses, and tree cavities, are abundant because of relatively low human disturbance (Jaroszewicz et al., 2019).
There is a long-standing history of mycobiota research in Białowieża Primeval Forest. The first studies of mycobiota in this area date back to the nineteenth century (Błoński, 1889; Błoński et al., 1888). Extensive inventory and taxonomic works in Białowieża National Park were conducted by various researchers in the twentieth century. Domański published a series of 18 papers entitled “Wood inhabiting fungi in Białowieża virgin forest” in Acta Societatis Botanicorum Poloniae between 1965 to 1972 (as cited in Karasiński & Wołkowycki, 2015). The dematiaceous hyphomycetes were investigated by Alina Borowska and her students, but the lists of species are available only as manuscripts (Kostecka, 1989; Szulc, 1995). An extensive and methodical study of lichens, including a summary of previous knowledge, was published by Cieśliński and Tobolewski (1988). The largest inventory project on the fungal diversity of this region, called CRYPTO, was conducted between 1987 and 1991. The project focused on one forest section (number 256) of Białowieża National Park and recorded 1,380 species of micro-, macrofungi, and lichens in an area of 144 ha (Bujakiewicz et al., 1992; Faliński & Mułenko, 1992, 1995, 1997). Knowledge about the lichenobiota of Białowieża Forest was first compiled in a red list of threatened lichens in Białowieża Old-Growth Forest (Czyżewska & Cieśliński, 2003), and later in a checklist of lichens of Białowieża Primeval Forest, which included 450 species (Cieśliński, 2010). Recently, numerous new species of lichens and allied fungi have been noted (e.g., Matwiejuk, 2011; Łubek & Jaroszewicz, 2012; Łubek et al., 2018).
An increasing number of studies are now using next-generation sequencing of amplicons to assess fungal diversity (Lindahl et al., 2013; Nilsson, Anslan, et al., 2019). Short sequence insufficiency for species recognition and a shortage of reference sequences in the databases used to be limiting for the application of this method (Nilsson, Anslan, et al., 2019), but improvements in the coverage of the UNITE database (Nilsson, Larsson, et al., 2019) and advances in sequencing technology are very promising. A recent estimate of fungal diversity based on various methods concluded that 2.2. to 3.8 million fungal species could inhabit our planet (Hawksworth & Lücking, 2017). However, only 5,317 generic names of fungi are represented in the GenBank (November 12, 2020) NCBI database (Genbank; Hawksworth & Lücking, 2017). This highlights the importance of generating reference barcode sequences for rare or endemic fungal species, including old-growth forest species, which are scarcely represented in internal transcribed spacer (ITS) sequence records in GenBank.
The Global Biodiversity Information Facility (GBIF; https://www.gbif.org/) is currently the most widely used database for all kinds of biodiversity studies but exhibits similar limitations. The database currently contains 19,389,938 fungal observations representing 154,183 species. However, there are only 2,481 fungal records from the Polish part of Białowieża Forest. Of these, most (543 records) came from the iNaturalist citizen science application (https://www.inaturalist.org/).
Rapid biodiversity inventories, called bioblitz, are a means to teach people about natural sciences and species in the field, while also raising awareness about the need for nature conservation. Bioblitzes generate important biodiversity datasets, especially if many expert naturalists (amateur or professional) are involved (Parker et al., 2018). Such inventories, called expert bioblitzes by Parker et al. (2018), have a long tradition in the Polish mycological community. Short field trips and fieldwork organized by the Mycological Section of the Polish Botanical Society, the Polish Mycological Society, national parks, museums, and amateur naturalist groups (e.g., bio-forum.pl; https://www.bio-forum.pl/), are rich sources of specimens and biodiversity data (e.g., Kujawa et al., 2015, 2018). Such rapid fungal inventories can record several hundred species from various taxonomic groups. However, the number of species recorded is highly dependent on the weather conditions and the specialists involved (e.g., Gierczyk et al., 2013; Kujawa et al., 2018; Ruszkiewicz-Michalska et al., 2015). Mycological meetings, like international congresses, offer unique opportunities to involve a large group of expert mycologists in species diversity studies. The first of these inventories was performed during the IV Congress of European Mycologists (Warsaw) in 1966 and resulted in the first exhibition of fungi from Białowieża Forest. Beginning in 1993, the current tradition of the annual mushroom exhibition of Białowieża Primeval Forest has continued. Recently, Kujawa et al. (2018) reviewed and analyzed all historical records of nonlichenized fungi from this region, including the data gathered from these annual exhibitions. The records were based on macro- and micromorphological observations of the collected specimens. Combining these data, Kujawa et al. reported 1,144 macrofungal taxa, of which 85 taxa were new to Poland. Altogether, approximately 2,200 species of macrofungi are known from the Polish part of Białowieża Forest (Kujawa, 2020; Kujawa, Ślusarczyk, et al., 2020). At the 25th Jubilee exhibition in September 2019, 159 species of fungi were displayed and an additional 51 species were recorded. Among these were three species new to Poland (Athelopsis subinconspicua, Hypomyces microspermus, and Ophiocordyceps variabilis) and seven species new to Białowieża Forest (Kujawa, Ślusarczyk, et al., 2020).
Here, we report the diversity of fungi (including lichens and lichenicolous organisms) of Białowieża Primeval Forest as a result of a joint collection and identification effort from all participants of the 18th Congress of European Mycologists (CEM) in September 2019. The opportunity was seized to gather the diverse expertise of more than 200 international mycologists to collect and identify fungi using macro- and microscopic morphology, along with sequence data that has been made available in the GenBank and GBIF databases.
Material and Methods
Fungi were collected from managed areas of Białowieża Primeval Forest (Podlaskie Voivodeship, Poland) between September 19 and September 27, 2019 by participants of the 18th CEM and University of Warsaw students. Forest divisions 424 and 362 were sampled between September 19 and September 21, 2019 and divisions 403, 450A, 469C, and 491B between September 23 and September 27, 2019 (Figure 1). Sampled sites represented lime-oak-hornbeam forest habitat with the exception of divisions 469C and 491B, where secondary mixed forest prevailed (Forest Data Bank, 2020; Matuszkiewicz, 2002). Additionally, observations from excursions to Białowieża National Park were added. Observations made by iNaturalist are available on the 18th CEM project website: https://www.inaturalist.org/projects/xviiicem. iNaturalist records were manually checked for redundant observations and filtered out. All localities include a reference to the forest district and division if recorded from the managed part of Białowieża Forest (Figure 1).
Contour map of the Polish part of Białowieża Primeval Forest showing forest subsections. Białowieża National Park is shown in dark gray. Dots represent records of fungi. Names indicate nearby settlements.
The nomenclature was aligned using the GBIF species lookup tool (https://www.gbif.org/tools/species-lookup), and later corrected to follow Mycobank (https://mycobank.org/; Robert et al., 2013). If the current name from Mycobank is different from GBIF or one in used checklists, the latter are given as synonyms in brackets. For selected specimens, morphological identification was confirmed by sequencing of ITS rDNA. The sequences obtained in this study were deposited in the International Nucleotide Sequence Database Collaboration under the following GenBank accession numbers: MT229985–MT229993, MT240483, and MT240484. Voucher specimens were deposited in the Herbarium of the Faculty of Biology, University of Warsaw (WA) and the Herbarium of Swiss Federal Institute of Technology in Zurich (ZT Myc). The data on species occurrences were also deposited in GBIF (Siedlecki & Pawłowska, 2020). All species records we report here are also available in the supplementary Table S1.
Finally, the protective status of all species were annotated according to the regulation of the Polish Minister of the Environment (Minister of Environment, 2014), the “Red list of the macrofungi in Poland” (Wojewoda & Ławrynowicz, 2006), the IUCN Red List (The IUCN Red List of Threatened Species, 2020), the Checklist of Polish Larger Basidiomycetes (Wojewoda, 2003), A Preliminary Checklist of Micromycetes in Poland (Mułenko et al., 2008), the Checklist of Microfungi and Larger Ascomycetes of Białowieża Forest (Kozłowska et al., 2019), “The lichens of Poland. A fourth checklist” (Fałtynowicz & Kossowska, 2016), the “Red list of extinct and threatened lichens in Poland” (Cieśliński et al., 2003), and a literature database of macrofungi in Poland (Kujawa, 2020).
Results
The eight (three plus five) day inventory yielded 101 collections and 68 observations representing 142 species. During the 11 days of the dedicated bioblitz project on iNaturalist, 750 observations representing 223 species were recorded, out of which 418 observations were of research grade. After removing redundant observations and doubtful identifications, 392 records representing 161 species were obtained from iNaturalist (only these data will be further included in the current paper). In total, 561 observations representing 233 species were recorded. ITS rDNA sequences were obtained for 11 specimens: Crucibulum crucibuliforme, Mycena inclinata, Nectria cinnabarina, Pholiota sp., Postia stiptica, Steccherinum fimbriatum, Stereum subtomentosum, Trichaptum abietinum, Tremella aurantia, Tyromyces chioneus, and Xylaria sp.
In this study, we found nine strictly protected (SP) species in Poland (Amylocystis lapponica, Chrysothrix candelaris, Hapalopilus croceus, Melanohalea olivacea, Peltigera praetextata, Ramalina farinacea, Rhodotus palmatus, Skeletocutis odora, and Thelotrema lepadinum), and five partially protected (PP) species [Fistulina hepatica, Hericium coralloides, Hypogymnia tubulosa, Rhodofomes roseus (syn. Fomitopsis rosea), and Tuckermanopsis chlorophylla]. Additionally, we noted 49 fungal species listed on the “Red list of the macrofungi in Poland” in the following threat categories: one extinct (Ex) (Phlebia subochracea), 17 endangered (E), 19 rare (R), and 13 vulnerable (V). We also found one critically endangered (CR) species, Melanohalea olivacea, listed in the “Red list of threatened lichens in Białowieża Old-Growth Forest.” Furthermore, five species are listed in the IUCN Red List in the following threat categories: data deficient (DD), Resinoporia piceata; least-concern (LC), Amylocystis lapponica and Gomphidius glutinosus; vulnerable (VU), Hapalopilus croceus and Rhodotus palmatus. Finally, our study resulted in the discovery of four species new for Poland, Bryocentria brongniartii, Tremella coppinsii, Tremella hypocenomycis, and Zevadia peroccidentalis, and eight species that were not previously reported in Białowieża Primeval Forest, Hypomyces chrysostomus, Hypomyces rosellus, Lachnellula resinaria, Peniophora lycii, Phellinus viticola, Phlebia subochracea, Pronectria anisospora, and Typhula quisquiliaris.
All of the species noted for the first time from the Białowieża region or even from Poland form small fruit bodies; therefore, they were probably overlooked during previous investigations. Many fungal species are hard to identify or inconspicuous, and are thus left unrecorded by nonspecialists. This often results in an expert-distribution record of some taxa rather than species distribution.
The following abbreviations are used in the list of species. Location: BNP – Białowieża National Park; f. dist. – forest district; div. – divison; Str. Res. – former Strict Reserve of BNP. Record character: iN – iNaturalist record (see supplementary Table S1); GB – GenBank accession number; WA – University of Warsaw Herbarium number; ZT Myc – Herbarium of Swiss Federal Institute of Technology in Zurich; * – taxon new for Białowieża Primeval Forest; ** – taxon new for Poland. Protective status: Ex, CR, E, V, R – category of threat according to the “Red list of the macrofungi in Poland” or “Red list of threatened lichens in the Białowieża Old-Growth Forest”; DD, VU, LC – category of threat according to the IUCN Red List of Threatened Species; PP – partially protected species; SP – strictly protected species. Names of collectors and/or identifiers: 18thCEM – 18th Congress of European Mycologists participants; BSI – Beatrice Senn-Irlet; CH – Christoffer Harder; DM – Diana Meiere; DS – Dmitry Schigel; FM UW, University of Warsaw Field Mycology course participants; HF – Howard Fox; ID - Inita Daniele; IKG – Irmgard Krisai-Greilhuber; JP – Julia Pawłowska; MC – Maria Cullen; MG – Michał Gorczak; MO – Martina Oberhofer; MW – Marta Wrzosek.
Ascomycota
Aleuria aurantia (Pers.) Fuckel, BNP, Str. Res., 52.7218N, 23.9069E, 2019-09-21, iN:33129946; f. dist. Białowieża, div. 403, 52.7217N, 23.9071E, 2019-09-24, iN:33326108.
Ascocoryne sarcoides (Jacq.) J. W. Groves & D. E. Wilson, BNP, Str. Res., 52.7511N, 23.8672E, 2019-09-19–2019-09-20, det. HF & MC, vid. HF & MC; BNP, Str. Res., 52.7211N, 23.8365E, 2019-09-27, iN:33475021.
Bisporella citrina (Batsch) Korf & S. E. Carp., f. dist. Hajnówka, div. 362, 52.7269N, 23.7134E, 2019-09-20, iN:33070323.
** Bryocentria brongniartii (P. Crouan & H. Crouan) Döbbeler, f. dist. Białowieża, div. 424, 52.7084N, 23.7879E, 2019-09-20, leg. 18thCEM, det. BSI, ZT Myc 60832; Figure 2D–F. Notes:Bryocentria brongniartii is a representative of the Bionectriaceae family which is characterized by the formation of small, orange perithecia. It parasites on liverworts, such as Frullania (Döbbeler, 2004, 2010) and is known from the northern hemisphere. There are 26 occurrences from Austria, Germany, Great Britain, Ireland, Italy, Norway, Spain and the USA reported in GBIF (2020).
* Hypomyces chrysostomus Berk. & Broome [syn. Sporophagomyces chrysostomus (Berk. & Broome) K. Põldmaa & Samuels], f. dist. Browsk, div. 78, 52.8243N, 23.7341E, 2019-09-20, iN:33056979. Notes:Hypomyces chrysostomus is a fungicolous ascomycete growing on Ganoderma spp, Fomes conchatus, and Rigidoporus microporus (Rogerson & Samuels, 1993), and it is known from South Asia, North and South America. In Europe, H. chrysostomus has been recorded from Belgium, Denmark, Estonia, and Germany (GBIF, 2020). In Poland, it has been recorded recently from Knyszyn Forest (as Acremonium lindtneri) (Kujawa et al., 2019), previously it was noted at least twice on mycological website bio-forum.pl (Domian, 2006; Drzewiecki, 2016) and additionally once, incorrectly as anamorph of Ganoderma lucidum from Biebrza National Park (Kujawa et al., 2012).
Kretzschmaria deusta (Hoffm.) P. M. D. Martin, BNP, Str. Res., 52.7267N, 23.9231E, 2019-09-23, iN:33261346; f. dist. Białowieża, div. 424, 52.7084N, 23.7879E, 2019-09-20, leg. 18thCEM, det. IKG, WA0000072741.
* Lachnellula resinaria (Cooke & W. Phillips) Rehm, f. dist. Białowieża, div. 424, 52.7084N, 23.7879E, 2019-09-20, leg. 18thCEM, det. BSI. Notes: Although L. resinaria is not protected, red-listed, or new to Poland, it is known from only one historical record in this country. The species was previously reported in 1908 by Schroeter from the Silesia region on the bark of Abies spp. (Schroeter, 1908). This observation in Białowieża Primeval Forest is therefore a significant finding.
Xanthoria parietina (L.) Beltr., BNP, Palace Park, 52.7060N, 23.8467E, 2019-09-19–2019-09-20, det. HF & MC, vid. HF & MC; Białowieża village, 52.6932N, 23.8327E, 2019-09-18, iN:32943403; Białowieża village, 52.6997N, 23.8449E, 2019-09-24, iN:33318128; Białowieża village, 52.7031N, 23.8571E, 2019-09-26, iN:33417221.
Lichenicolous Fungi
Athelia arachnoidea (Berk.) Jülich, on Lepraria incana (L.) Ach. sward on Tilia cordata Mill., BNP, Palace Park, 52.7060N, 23.8467E, 2019-09-19, det. HF, vid. HF & MC.
Clypeococcum hypocenomycis D. Hawksw., on Hypocenomyce scalaris (Ach. ex Lilj.) M. Choisy, BNP, Palace Park, 52.7060N, 23.8467E, 2019-09-19–2019-09-20, det. HF, vid. HF & MC.
Lichenoconium erodens M. S. Christ. & D. Hawksw., on Hypogymnia physodes (L.) Nyl., BNP, Str. Res., 52.7511N, 23.8672E, 2019-09-19–2019-09-20, det. HF & MC, vid. HF & MC.
Lichenostigma maureri Hafellner, on Pseudevernia furfuracea (L.) Zopf on Larix decidua Mill. branches, BNP, Palace Park, 52.706N, 23.8467E, 2019-09-19, det. HF, vid. HF & MC.
* Pronectria anisospora (Lowen) Lowen, on Hypogymnia physodes (L.) Nyl. on Pinus sp. bark, BNP, Palace Park, 52.7060N, 23.8467E, 2019-09-19, det. HF, vid. HF & MC. Notes:Pronectria anisospora, which grows on Hypogymnia physodes (Khodosovtsev et al., 2012; Lowen, 1990), is a Northern-Hemisphere species with more than 100 known localities mainly in Estonia, Germany, Great Britain, Ireland, Norway, and the USA (GBIF, 2020). In Poland, it was noted only once from Drawieński National Park (NW Poland, Western Pomerania) as Trichonectria anisopora (Schiefelbein et al., 2012).
** Tremella coppinsii Diederich & G. Marson, on Platismatia glauca (L.) W. L. Culb. & C. F. Culb., BNP, Str. Res., 52.7511N, 23.8672E, 2019-09-19–2019-09-20, det. HF, vid. HF & MC. Notes:Tremella coppinsii, although originally described from Sarawak, Malaysia (Diederich & Marson, 1988) is also known from Belgium, Estonia, Ireland, Norway, Spain, Sweden, the UK and the USA (Ertz & Diederich, 2008; Fox, 2001; Millanes et al., 2014; Sérusiaux et al., 2006). This species is associated exclusively with Platismatia glauca.
** Tremella hypocenomycis Diederich, on Hypocenomyce scalaris (Ach. ex Lilj.) M. Choisy, BNP, Palace Park, 52.7060N, 23.8467E, 2019-09-19–2019-09-20, det. HF, vid. HF & MC. Notes: An extremely rare T. hypocenomycis is restricted to Hypocenomyce scalaris and is previously only known from two specimens from Finland (Pippola & Kotiranta, 2008).
** Zevadia peroccidentalis J. C. David & D. Hawksw., on Usnea sp., BNP, Str. Res., 52.7511N, 23.8672E, 2019-09-19–2019-09-20, det. HF & MC, vid. HF & MC. Notes:Zevadia peroccidentalis, a lichenicolous fungus associated with Usnea spp. was, until now, only known from Ireland (David & Hawksworth, 1995; Fox, 2001).
Basidiomycota
Abortiporus biennis (Bull.) Singer, BNP, Str. Res., 52.7233N, 23.8482E, 2019-09-23, iN:33264250; E.
Amanita citrina Pers., f. dist. Białowieża, div. 451, 52.7011N, 23.8161E, 2019-09-23–2019-09-27, leg. FM UW, det. MW & JP, WA0000072785.
Amylocystis lapponica (Romell) Bondartsev & Singer, BNP, Str. Res., 52.7573N, 23.9063E, 2019-09-22, iN:33195620; BNP, Str. Res., 52.7230N, 23.8513E, 2019-09-23, iN:33264267; BNP, Str. Res., 52.7420N, 23.8315E, 2019-09-24, iN:33316063; SP, E, LC. Notes:Amylocystis lapponica, a polypore which grows on the logs of Norway spruce in old-growth humid forests is strictly protected in Poland (Minister of Environment, 2014). It is red-listed in Poland, with high threat category – E (Wojewoda & Ławrynowicz, 2006). Although the species has only been observed in three localities in the country (Kujawa, Gierczyk, & Ślusarczyk, 2020; Wojewoda, 2003), there have been more than four thousand known observations around the world (GBIF, 2020). Therefore, its threat category on the IUCN Red List is LC (least-concern) (The IUCN Red List of Threatened Species,2020).
Amylostereum areolatum (Chaillet ex Fr.) Boidin, BNP, Str. Res., 52.7575N, 23.9069E, 2019-09-22, iN:33195364.
Antrodiella serpula (P. Karst.) Spirin & Niemelä, BNP, Str. Res., 52.7260N, 23.8754E, 2019-09-23, iN:33264292; R.
Artomyces pyxidatus (Pers.) Jülich, BNP, Str. Res., 52.7232N, 23.9070E, 2019-09-21, iN:33129349; f. dist. Białowieża, div. 424, 52.7084N, 23.7879E, 2019-09-20, leg. 18thCEM, det. BSI, WA0000072714; V.
Auricularia mesenterica (Dicks.) Pers., BNP, Str. Res., 52.7193N, 23.8429E, 2019-09-20, iN:33057646; f. dist. Białowieża, div. 424, 52.7060N, 23.7867E, 2019-09-20, iN:33060568; f. dist. Białowieża, div. 425, 52.7092N, 23.8000E, 2019-09-23, iN:33250849; f. dist. Białowieża, div. 424, 52.7084N, 23.7866E, 2019-09-26, iN:33428918; f. dist. Białowieża, div. 424, 52.7084N, 23.7879E, 2019-09-20, leg. 18thCEM, det. MG, WA0000072721; R.
Calcipostia guttulata (Sacc.) B. K. Cui, L. L. Shen & Y. C. Dai [syn. Postia guttulata (Peck) Jülich], f. dist. Białowieża, div. 428, 52.7130N, 23.9001E, 2019-09-24, iN:33306063.
Calocera cornea (Batsch) Fr., f. dist. Białowieża, div. 451, 52.6970N, 23.8191E, 2019-09-24, iN:33303269; f. dist. Białowieża, div. 403, 52.7180N, 23.9140E, 2019-09-24, iN:33322328; f. dist. Białowieża, div. 424, 52.7095N, 23.7875E, 2019-09-26, iN:33428980; BNP, Str. Res., 52.7212N, 23.8375E, 2019-09-27, iN:33486560.
Calocera furcata (Fr.) Fries, BNP, Str. Res., 52.7511N, 23.8672E, 2019-09-19–2019-09-20, det. HF & MC, vid. HF & MC; R.
Fomitopsis betulina (Bull.) B. K. Cui, M. L. Han & Y. C. Dai, f. dist. Browsk, div. 98, 52.8175N, 23.7260E, 2019-09-20, iN:33056891; f. dist. Białowieża, div. 425, 52.7094N, 23.8014E, 2019-09-23, iN:33252379; BNP, Str. Res., 52.7158N, 23.8375E, 2019-09-25, iN:33367076; f. dist. Białowieża, div. 469, 52.6916N, 23.7171E, 2019-09-25, iN:33369018; f. dist. Białowieża, div. 424, 52.7084N, 23.7879E, 2019-09-20, leg. 18thCEM, det. DM, WA0000072754.
Fomitopsis pinicola (Sw.) P. Karst., BNP, Palace Park, 52.706N, 23.8467E, 2019-09-19–2019-09-20, det. HF & MC, vid. HF & MC; BNP, Palace Park, 52.7088N, 23.8474E, 2019-09-19, iN:33051687; BNP, Palace Park, 52.7032N, 23.8466E, 2019-09-20, iN:33057230; BNP, Str. Res., 52.7228N, 23.8441E, 2019-09-19, iN:33028856; BNP, Str. Res., 52.7177N, 23.8429E, 2019-09-20, iN:33053475; BNP, Str. Res., 52.7473N, 23.9071E, 2019-09-22, iN:33193338; BNP, Str. Res., 52.7274N, 23.9244E, 2019-09-23, iN:33261512; BNP, Str. Res., 52.7239N, 23.8446E, 2019-09-23, iN:33263860; BNP, Str. Res., 52.7614N, 23.8555E, 2019-09-24, iN:33317257; BNP, Str. Res., 52.7801N, 23.8591E, 2019-09-24, iN:33317961; f. dist. Browsk, div. 78, 52.8232N, 23.7329E, 2019-09-20, iN:33056680; f. dist. Hajnówka, div. 362, 52.7252N, 23.7104E, 2019-09-20, iN:33069350; f. dist. Hajnówka, div. 362, 52.7280N, 23.7143E, 2019-09-20, iN:33076264; f. dist. Białowieża, div. 425, 52.7119N, 23.8040E, 2019-09-23, iN:33250282; f. dist. Białowieża, div. 450, 52.6987N, 23.8020E, 2019-09-23, iN:33252419; f. dist. Białowieża, div. 451, 52.6959N, 23.8220E, 2019-09-24, iN:33303011; f. dist. Białowieża, div. 403, 52.7181N, 23.9118E, 2019-09-24, iN:33303186; f. dist. Białowieża, div. 451, 52.6971N, 23.8189E, 2019-09-24, iN:33303230; Białowieża village, 52.6985N, 23.8468E, 2019-09-24, iN:33377072; f. dist. Białowieża, div. 469, 52.6883N, 23.7183E, 2019-09-25, iN:33360650; f. dist. Białowieża, div. 424, 52.7084N, 23.7879E, 2019-09-20, leg. 18thCEM, det. DM, WA0000072746.
Hapalopilus croceus (Pers.) Bondartzew & Singer, BNP, Str. Res., 52.7619N, 23.9075E, 2019-09-22, iN:33193945; BNP, Str. Res., 52.7233N, 23.8480E, 2019-09-23, iN:33264240; f. dist. Białowieża, div. 450, 52.7012N, 23.7996E, 2019-09-23–2019-09-27, leg. FM UW, det. MW & JP, WA0000072790; SP, E, VU. Notes:Hapalopilus croceus is the orange bracket fungus from the order Polyporales. It grows almost exclusively on old oaks (Quercus spp.) (Dahlberg, 2019). According to GBIF, more than 800 occurrences were reported, mainly from the Northern Hemisphere (GBIF, 2020). In Poland, this species is strictly protected (Minister of Environment, 2014) and is red-listed in Poland, with high threat category – E (Wojewoda & Ławrynowicz, 2006). The species is known from less than 10 localities in the country (Kujawa, 2020; Kujawa, Gierczyk, & Ślusarczyk, 2020).
Hapalopilus nidulans (Fr.) P. Karst [syn. Hapalopilus rutilans (Pers.) Murrill], BNP, Str. Res., 52.7180N, 23.8404E, 2019-09-20, iN:33054439; f. dist. Białowieża, div. 448, 52.6996N, 23.7752E, 2019-09-26, iN:33418400.
Hericium coralloides (Scop.) Pers., f. dist. Białowieża, div. 424, 52.7112N, 23.7885E, 2019-09-26, iN:33428998; V, PP.
Postia tephroleuca (Fr.) Jülich, f. dist. Browsk, div. 78, 52.8226N, 23.7322E, 2019-09-20, iN:33056728; f. dist. Browsk, div. 98, 52.8199N, 23.7286E, 2019-09-20, iN:33056920; BNP, Str. Res., 52.7337N, 23.8749E, 2019-09-23, iN:33264299.
Pycnoporellus fulgens (Fr.) Donk, BNP, Str. Res., 52.7180N, 23.8402E, 2019-09-20, iN:33054381; f. dist. Browsk, div. 78, 52.8219N, 23.7325E, 2019-09-20, iN:33115008; V.
Resinoporia piceata (Runnel, Spirin & Vlasák) Audet (syn. Antrodia piceata K. Runnel, Spirin & Vlasák), BNP, Str. Res., 52.7240N, 23.8445E, 2019-09-23, iN:33263834; BNP, Str. Res., 52.7575N, 23.8450E, 2019-09-24, iN:33316961; DD. Notes: IUCN red-listed taxon, Resinoporia piceata, is a wood-decaying polypore. It forms resupinate basidiomata on fallen trunks of coniferous trees, mainly in old-growth forests. There are only ca. 60 known localities of this fungus worldwide (Kunca, 2019). In Poland, R. piceata was reported only from Białowieża Primeval Forest (Karasiński & Wołkowycki, 2015).
Rhodofomes roseus (Alb. & Schwein.) Vlasák, f. dist. Browsk, div. 98, 52.8201N, 23.7291E, 2019-09-20, iN:33056955; BNP, Palace Park, 52.7033N, 23.8462E, 2019-09-20, iN:33093394; f. dist. Browsk, div. 78, 52.8234N, 23.7324E, 2019-09-20, iN:33115189; f. dist. Białowieża, div. 450, 52.6992N, 23.8034E, 2019-09-23, iN:33252442; BNP, Str. Res., 52.7582N, 23.9074E, 2019-09-22, iN:33194910;BNP, Str. Res., 52.7183N, 23.8403E, 2019-09-20, iN:33054664; BNP, Str. Res., 52.7251N, 23.9157E, 2019-09-23, iN:33260654; BNP, Str. Res., 52.7240N, 23.8448E, 2019-09-23, iN:33263850; BNP, Str. Res., 52.7578N, 23.8448E, 2019-09-24, iN:33317034; PP, E.
Rhodotus palmatus (Bull.) Maire, BNP, Str. Res., 52.7599N, 23.9072E, 2019-09-22, iN:33194742; SP, E, VU. Notes:Rhodotus palmatus has been assigned the category threat of near threatened (NT) in the global IUCN assessment. However, at the European level, the species is classified as vulnerable (VU) (Iršėnaitė et al., 2019). Moreover, it is on the Red List of macrofungi in Poland with the category of threat E (Wojewoda & Ławrynowicz, 2006). Rhodotus palmatus is known to form fruitbodies on dead trunks of deciduous trees (particularly on Ulmus spp., Fraxinus spp., and Fagus spp.) in moist habitats (Iršėnaitė et al., 2019). In Poland, R. palmatus is known from two localities only (Kujawa, Gierczyk, & Ślusarczyk, 2020).
* Typhula quisquiliaris (Fr.) Henn., f. dist. Hajnówka, div. 468, 52.6857N, 23.7159E, 2019-09-25, iN:33322387; R. Notes:Typhula quisquiliaris is a minuscule, white club fungus from the family Typhulaceae (Agaricales) that usually grows on Pteridium aquilinum petioles. In Poland, the species was observed for the first time in 1973 in Katowice (Silesia) by Wojewoda (2000) and is also known from Kashubian Landscape Park (Pomerania) (Karasiński, 2016). However, reliable data on its distribution in Poland are lacking. Typhula quisquiliaris is likely overlooked due to its small size and specific substrate requirement and could be much more prevalent than indicated by current estimates.
Tyromyces chioneus (Fr.) P. Karst., f. dist. Białowieża, div. 424, 52.7084N, 23.7879E, 2019-09-20, leg. 18thCEM, det. IKG, GB: MT229985, WA0000072730.
A rapid inventory carried out during 18th Congress of European Mycologists (September 18–29, 2019) yielded 101 collections and 68 observations representing 142 species. A dedicated accompanying bioblitz project on iNaturalist resulted in 392 unique, research grade records representing 161 species. In total, 561 observations representing 233 species were recorded. Four species new to Poland (Bryocentria brongniartii, Tremella coppinsii, T. hypocenomycis, and Zevadia peroccidentalis) and eight species new to Białowieża Primeval Forest (Hypomyces chrysostomus, Hypomyces rosellus, Lachnellula resinaria, Peniophora lycii, Phellinus viticola, Phlebia subochracea, Pronectria anisospore, and Typhula quisquiliaris) were recorded.
Supporting Material
The following supporting material is available for this article:
Table S1. Detailed table of all records with metadata.
Handling Editor
Małgorzata Ruszkiewicz-Michalska; Institute for Agricultural and Forest Environment, Polish Academy of Sciences, Poland; University of Łódź, Poland; https://orcid.org/0000-0001-8901-0552
Authors’ Contributions
JP initiated the study and together with MG wrote the first draft of the manuscript; IS, MG, and MK coordinated specimens collection, sampling and preservation. MG, MC, ID, HF, CH, JK, DM, IKG, DW, MO, DS, BSI, MW, and JP identified recorded fungi; JP, IS, MM, DS, DW, and MK were responsible for data management; IS and ZB generated ITS barcode sequences; DS organized and coordinated the iNaturalist project; all authors contributed to the preparation of the manuscript
Competing Interests
No competing interests have been declared.
Acknowledgments
We are grateful to the director of Białowieża National Park, Dr. Michał Krzysiak as well as to Ewa Moroz-Keczyńska, Dr. Joanna Bober, Hanna Schmidt and other employees of Białowieża National Park for their help and for offering their facilities. We are grateful to all 18th CEM participants and students of the field mycology course who collected specimens and participants and identifiers of the iNaturalist project. We also acknowledge students of mycology classes who collected specimens and performed part of molecular identification: Miłosz Augustyniak, Monika Babis, Bartosz Bałdowski, Maciej Biela, Laura Bielawska, Justyna Borowiecka, Małgorzata Chwalińska, Zofia Cieślińska, Julia Cyran, Urszula Drwęcka, Agnieszka Gajewska, Michał Gierek, Sebastian Korab, Karolina Koszarska, Grzegorz Kowalczyk, Agnieszka Kuc, Marharyta Lyzohub, Agata Michalak, Patryk Mierzejewski, Wojciech Pawlak, Emilia Pielaszek, Zuzanna Pyffel, Albert Roethel, Joanna Rosińska, Marta Sałek, Aleksandra Sobkowiak, Ida Tymińska, Patryk Walędziak, Grzegorz Wałpuski, Eliza Żurawska. Finally, we would like to specially thank Dr. Anna Kujawa for many critical remarks to the manuscript and sharing unpublished data.
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