Biomass, abundance and sensitivity to antibiotics and antimycotics of the fungi in the Vistula River with its main tributaries

Anna Pietryczuk, Andrzej S. Górniak, Adam Więcko, Adam Cudowski

Abstract


Mycoplankton of Vistula River and its main tributaries biomass as well as the number and morphotype diversity was studied in summer and autumn 2011. Summer mycoplankton biomass was within the range of 0.2 – 0.5 μg/l, while in the autumn it was two times wider range (0.1 – 1.3μg/l). The number of fungi in river water most often did not exceed 1000 – 2000 CFU/ml. Fungi colonies isolated from rivers water were sensitive to the commonly used amphotericine B (10 μg) and gentamicin (10 μg). It seems to be plausible that aquatic fungi can acquire immunity to drugs as a result of horizontal transfer of a gene responsible for drug resistance or as an effect of antibiotics and antimycotics getting into the aquatic ecosystems from wastewaters.

Keywords


river; aquatic fungi; antibiotics; antimycotics

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References


Anaissie E.J., Penzak S.R., Dignani M.C. 2002. The hospital water supply as a source of nosocomial infections. A plea for action. Archv. Internal Med. 162: 1483-1492.

Anon. 2003. Drinking water regulations (in Swedish). SLVFS 2001: 30. National Food Administration, Stockholm, Sweden.

Bossche H.V., Marichal P., Odds F.C. 1994. Molecular mechanisms of drug resistance in fungi. Trends Microbiol. 2: 393-400.

Cabral D., Fernández P. 2002. Fungal spoilage of bottled mineral water. Intern. J. Food Microbiol. 72: 73-76.

Canhoto C., Graca M.A.S. 2008. Interactions between fungi and stream invertebrates: back to the future, Novel techniques and ideas in mycology, Fungal diversity research series (Sridhar K.R., Bärlocher F., Hyde K.D., eds), pp. Fungal Diversity Press, Yunnan, P.R. China: 305-325.

Davis M.W., Lamar, R. T. 1992. Evaluation of methods to extract ergosterol for quantitation of soil fungal biomass. Soil Biol Biochem. 24 (3): 189-198.

Descals E. 2007. Techniques for handling ingoldian fungi. (In:) M.A.S. Graça, F. Bärlocher, M.O. Gessner (eds). Methods to study litter decomposition. A practical guide. Springer: 129-141.

Doggett M.S. 2000. Characterization of fungal biofi lms within a municipal water distribution system. Appl. Environ. Microbiol. 66: 1249-1251. http://dx.doi.org/10.1128/AEM.66.3.1249-1251.2000

Flannigan B., McCabe E.M., McGarry F. 1991. Allergenic and toxigenic microorganisms in houses. J. Appl. Bacteriol. Symp. Suppl. 70: 61S-73S.

Gotz A., Smalla K. 1997. Manure enhances plasmid mobilization and survival in Pseudomonas putida introduced into the fi eld soil. Appl. Environ. Microbiol. 63: 1980-1986.

Gruszecki W.I., Luchowski R., Gagoś M., Arczewska M., Sarkar P., Hereć M., Myśliwa-Kurdziel B., Strzałka K., Gryczynski I., Gryczynski Z. 2009. Molecular organization of antifungal antibiotic amphotericin B in lipid monolayers studied by means of Fluorescence Lifetime Imaging Microscopy. Biophysics Chem. 143: 95-101.

Hageskal G., Knutsen A.K., Gaustad P., de Hoog G.S., Skaar I. 2006. Diversity and signifi cance of mold species in Norwegian drinking water. Appl. Environ. Microbiol. 72: 7586-7593. http://dx.doi.org/10.1128/AEM.01628-06

Hageskal G., Gaustad P., Heier B.T., Skaar I. 2007. Occurrence of moulds in drinking water. J. Appl. Microbiol. 102: 774-780. http://dx.doi.org/10.1111/j.1365-2672.2006.03119.x

Hageskal G., Lima N., Skaar I. 2009. The study of fungi in drinking water. Mycol. Res. 113: 165-172. http://dx.doi.org/10.1016/j.mycres.2008.10.002

Hill K.E., Fry J.C., Weightman A.J. 1994. Gene transfer in the aquatic environment: persistence and mobilization of the catabolic recombinant plasmid pD10 in the epilithon. Microbiol. 140: 1555-1563.

Ho W.H., To P.C., Hyde K.D. 2003. Induction of antibiotic production of freshwater fungi using mixculture fermentation. Fungal Diversity 12: 45-51.

Holt R.J. 1975. Laboratory tests of antifungal drugs. J. Clin. Path. 28: 767-774.

Jørgensen N.O.G., Stepanauskas R. 2009. Biomass of pelagic fungi in Baltic rivers. Hydrobiologia 623:105-112. http://dx.doi.org/10.1007/s10750-008-9651-2

Kim S., Aga D.S. 2007. Potential ecological and human health impact of antibiotics and antibiotic-resistant bacteria from wastewater treatment plants. J. Toxicol. Environ. Health 10: 559-573.

Krauss G.J., Solé M., Krauss G., Schlosser D., Wesenberg D., Bärlocher F. 2011. Fungi in freshwaters: ecology, physiology and biochemical potetntial. FEMS Microbial. Rev. 35: 620-651.

Kűmmerer K. 2009. The presence of pharmaceuticals in the environment due to human use: present knowledge and future challenges. J. Environ. Manage 90: 2354-2366.

López-Archilla A.I., González A.E., Terrón M.C., Amils R. 2004. Ecological study of the fungal populations of the acidic Tinto River in southwestern Spain. Can. J. Microbiol. 50: 923–934.

Marano A.V., Steciow M.M. 2006. Frequency and abundance of zoosporic fungi in some lotic environments of Buenos Aires Province (Argentina). J. Agr. Tech. 2(1): 17-28.

Miersch J., Grancharov K. 2008. Cadmium and heat response of the fungus Heliscus lugdunensis isolated from highly polluted and unpolluted areas. Amino Acids 34: 271-277.

Mille-Lindblom C., Tranvik L.J. 2003. Antagonism between bacteria and fungi on decomposing aquatic plant litter. Microb Ecol. 45: 173-182. http://dx.doi.org/10.1007/s00248-002-2030-z

Moore J.E., Rao J.R., Moore P.J.A., Millar B.C., Goldsmith C.E., Loughrey A., Rooney P.J. 2010. Determination of total antibiotic resistance in waterborne bacteria in rivers and streams in Northern Ireland: Can antibiotic-resistant bacteria be an indicator of ecological change? Aquat. Ecol. 44: 349-358.

Pascoal C., Cássio F., Marcotegui A., Sanz B., Gomes P. 2005. Role of fungi, bacteria and invertebrates in leaf litter breakdown in a polluted river. J N Am. Benthol. Soc. 24: 784-797.

Qi B., Moe W. 2002. Biodegradation of volatile organic compounds by fi ve fungal species. Appl. Microbiol. Bot. 58: 684-689.

Ribeiro A., Machado A.P., Kozakiewicz Z., Ryan M., Luke B,. Buddie A.G., Venăncio A. Lima N., Kelley J. 2006. Fungi in bottled water: a case study of production plants. Revista Iberoamericana de Micologia 23: 139-144.

Rufo P.A., Merlin D., Riegler M., Ferguson-Maltzman M.H., Dickinson B.L., Brugnara C., Alper S.L., Lencer W.I. 1997. The antifungal antibiotic, clotrimazole, inhibits chloride secretion by human intestinal T84 cells via blockade of distinct basolateral K+ conductances. Demonstration of effi cacy in intact rabbit colon and in an in vivo mouse model of cholera. J Clin. Invest. 100: 3111-3120.

Seena S., Wynberg N., Bärlocher F. 2008. Fungal diversity during leaf decomposition in a stream assessed through clone libraries. Fungal Divers. 30: 1-14.

Sridhar K.R., Sudheep N.M. 2010. Diurnal fl uctuation of spores of freshwater hyphomycetes in two tropical streams. Mycosphere 1: 89-101.

Wong M.K.M., Goh T.K., Hodgkiss I.J., Hyde K.D., Ranghoo V.M., Tsui C.K.M., Ho W.H., Wong W.S.W., Yuen T.K. 1998. Role of fungi in freshwater ecosystems. Biodiversity and Conservation 7: 1187-1206.

Zebouh M., Thomas C., Honderlick P., Lemee L., Segonds C., Wallet F., Husson M.O. 2008. Direct antimicrobial susceptibility testing method for analysis of sputum collected from patients with cystic fi brosis. J. Cyst. Fibros. 7: 238-243. http://dx.doi.org/10.1016/j.jcf.2007.10.002




DOI: https://doi.org/10.5586/am.2013.025

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