Genetic diversity of natural psammophilous populations of Hypogymnia physodes (L.) Nyl. on Polish seacoast dunes

Anetta Wieczorek, Magdalena Achrem, Aleksandra Truszkowska, Andrzej Łysko, Agnieszka Popiela


Hypogymnia physodes is a lichenized fungus of the family Parmeliaceae. The aim of this study was to compare the level of genetic diversity in eight psammophilous and three epiphytic populations of this species from the Baltic coast in Poland, based on randomly amplified polymorphic DNA (RAPD) markers. In the reactions with nine primers, 153 fragments were obtained, of which 133 were polymorphic. In one reaction, from 0 (for lich2 primer) to 55 (for C02 primer) amplicons were obtained. A Dice’s genetic similarity index matrix was constructed based on the results of RAPD marker polymorphism examination. The values of similarity indices ranged from 0.00 to 0.73. Results of this study confirm the separateness of all three epiphytic populations from those found on sand dunes (100% support, UPGMA/1000 trees).


genetic polymorphism; interpopulation variability; randomly amplified polymorphic DNA; RAPD; Hypogymnia physodes; sand dunes

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Printzen C, Ekman S. Genetic variability and its geographical distribution in the widely disjunct Cavernularia hultenii. Lichenologist. 2002;34:101–111.

Printzen C, Ekman S, Tønsberg T. Phylogeography of Cavernularia hultenii: evidence of slow genetic drift in a widely disjunct lichen. Mol Ecol. 2003;12(6):1473–1486.

Cassie DM, Piercey-Normore MD. Dispersal in a sterile lichen-forming fungus, Thamnolia subuliformis (Ascomycotina: Icmadophilaceae). Botany. 2008;86(7):751–762.

Mattsson JE, Hansson AC, Lindblom L. Genetic variation in relation to substratum preferences of Hypogymnia physodes. Lichenologist. 2009;41(5):547–555.

Cubero OF, Crespo A, Ochando MD. Aplicación de la técniqua de RAPD a estudios en líquenes. In: Proceedings of the XI Simposio Nacional de Botánica Criptogámica; 1995 Sep 18–21; Santiago de Compostela, Spain. Santiago de Compostela: University of Vigo, University of Santiago de Compostela, University of A Coruña; 1995. p. 231.

Lohtander K, Myllys L, Sundin R, Källersjö M, Tehler A. The species pair concept in the lichen Dendrographa leucophaea (Arthoniales): analyses based on ITS sequences. Bryologist. 1998;101(3):404–411.[404:TSPCIT]2.0.CO;2

Lumbsch H, Schmitt I. RAPD-PCR of lichens. In: Kranner IC, Beckett RP, Varma AK, editors. Protocols in lichenology. Berlin: Springer; 2002. p. 412–421. (Springer Lab Manuals).

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms – amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990;18:6531–6535.

Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA. 1979;76:5269–5273.

Pavlìček A, Hrdá Š, Flegr J. FreeTree freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biol (Praha). 1999;45:97–99.

Hampl V, Pavlíček A, Flegr J. Construction and bootstrap analysis of DNA fingerprinting-based phylogenetic trees with the freeware program FreeTree: application to trichomonad parasites. Int J Syst Evol Microbiol. 2001;51:731–735.

Molnár K, Farkas E. Depsides and depsidones in populations of the lichen Hypogymnia physodes and its genetic diversity. Ann Bot Fenn. 2011;48:473–482.

Dyer PS, Murtagh GJ. Variation in the ribosomal ITS-sequence of the lichens Buellia frigida and Xanthoria elegans from the Vestfold Hills, Eastern Antarctica. Lichenologist. 2001;33(2):151–159.

Yüzbaşıoğlu E, Halıcı MG, Karabacak M, Aksoy A. RAPD and ISSR markers indicate high genetic variation within Lobathallia radiosa in Turkey. Mycol Prog. 2011;10:219–228.

Junghans DT, Gomes EA, Guimarães WV, Barros EG, Araújo EF. Genetic diversity of the ectomycorrhizal fungus Pisolithus tinctorius based on RAPD-PCR analysis. Mycorrhiza. 1998;7(5):243–248.

Heibel E, Lumbsch HT, Schmitt I. Genetic variation of Usnea filipendula (Parmeliaceae) populations in western Germany investigated by RAPDs suggests reinvasion from various sources. Am J Bot. 1999;86:753–757.

Lindblom L, Ekman S. Genetic variation and population differentiation in the lichen-forming ascomycete Xanthoria parietina on the island Storfosna, central Norway. Mol Ecol. 2006;15:1545–1559.

Wieczorek A, Achrem M, Mitka J, Rogalski M, Werczyńska K. Genetic variability of the populations of Zwackhia viridis (Ach.) Poetsch & Schied (Lecanographaceae, lichenized Ascomycetes) in the eastern Poland; geographical versus habitat distance. Pol J Ecol. 2014;62:253–261.

Knaepkens G, Knapen D, Bervoets L, Hänfling B, Verheyen E, Eens M. Genetic diversity and condition factor: a significant relationship in Flemish but not in German populations of the European bullhead (Cottus gobio L.). Heredity. 2002;89:280–287.

Toro MA, Caballero A. Characterization and conservation of genetic diversity in subdivided populations. Philos Trans R Soc Lond. 2005;360:1367–1378.

Brodo IM. Substrate ecology. In: Ahmadjian V, Hale ME, editors. The lichens. New York, NY: Academic Press; 1973. p. 401–441.

Werth S, Wagner HH, Gugerli F, Holderegger R, Csencsics D, Kalwij JM, et al. Quantifying dispersal and establishment limitation in a population of an epiphytic lichen. Ecology. 2006;87:2037–2046.[2037:QDAELI]2.0.CO;2

Gaya E, Fernández-Brime S, Vargas R, Lachlan RF, Gueidan C, Ramírez-Mejía M, et al. The adaptive radiation of lichen-forming Teloschistaceae is associated with sunscreening pigments and a bark-to-rock substrate shift. Proc Natl Acad Sci USA. 2015;112(37):11600–11605.