The Effects of Sucrose, Silver Nitrate, Plant Growth Regulators, and Ammonium Nitrate on Microrhizome Induction in Perennially-Cultivated Ginger (Zingiber officinale Roscoe) From Hue, Vietnam

Nguyen Hoang An, Tran Thi Minh Chien, Ho Thi Hoang Nhi, Nguyen Thi Minh Nga, Tran Thien Phuc, Lam Thi Ngoc Thuy, Tong Van Bao Thanh, Phan Thi Thao Nguyen, Truong Thi Bich Phuong


The number of research on ginger microrhizome production is low, despite awareness of the drawbacks to the traditional method of cultivation and the known health benefits associated with ginger essential oils. We examined the effects of several factors on microrhizome induction in order to create a production protocol for the cultivar found in Hue, Vietnam. To determine the optimal conditions for ginger microrhizome production, different concentrations of sucrose, plant growth regulators, ammonium nitrate, and silver nitrate were investigated. Microrhizome fresh weight and diameter were increased to the maximum values with application of BAP (6-benzyl amino purine), NAA (α- naphthaleneacetic acid), IBA (indole-3-butyric acid), and a low ammonium nitrate concentration, with 0.433 g at 9.03 mm, 0.437 g at 9.73 mm, 0.478 g at 10.80 mm, and 0.449 g at 9.53 mm, respectively. Additionally, we demonstrated that kinetin has an inhibitory effect on microrhizome growth. The biggest microrhizomes were grown on MS media containing the optimal concentrations for each factor – 80 g/L sucrose, 1.9 mg/L AgNO3, 550 mg/L ammonium nitrate, 4 mg/L BAP, 6 mg/L NAA, and 4 mg/L IBA.


culture optimization; nitrogen quantities; phytohormones; carbon source

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Abbas, M., Aly, U., Taha, H., & Gaber, E. I. (2014). In vitro production of microrhizomes in ginger (Zingiber officinale Rosco). Journal of Microbiology, Biotechnology and Food Sciences, 4(2), 142–148.

Archana, C. P., Geetha, S. P., & Balachandran, I. (2013). Microrhizome and minirhizome production in three high yielding cultivars of ginger (Zingiber officinale Rosc.). International Journal of Current Microbiology and Applied Sciences, 2(10), 477–484.

Arteca, R. N. (1996). Plant growth substances: Principles and applications. Springer.

Bleecker, A. B., Estelle, M. A., Somerville, C., & Kende, H. (1998). Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana. Science, 241, 1086–1089.

de Lange, J. H., Willers, P., & Nel, M. I. (1987). Elimination of nematodes from ginger (Zingiber officinale Roscoe) by tissue culture. Journal of Horticultural Science, 62, 249–252.

Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics, 11, 1–42.

Goren, L., Mattoo, A. K., & Anderson, J. D. (1984). Ethylene binding during leaf development and senescence. Journal of Plant Physiology, 117, 243–248.

Hosoki, T., & Sagawa, Y. (1977). Clonal propagation of ginger (Zingiber officinale Roscoe) through tissue culture. HortScience, 12, 451–452.

Kambaska, K. B., & Santilata, S. (2009). Effect of plant growth regulator on micropropagation of ginger (Zingiber officinale Rosc.) cv-Suprava and Suruchi. Journal of Agricultural Science and Technology, 5(5), 271–280.

Koda, Y., & Okazawa, Y. (1983). Influences of environmental, hormonal and nutritional factors on potato tuberization in vitro. Japanese Journal of Crop Science, 52(4), 582–591.

Mauk, C. S., & Langille, A. K. (1978). Physiology of tuberization in Solanum tuberosum L. cis-zeatin riboside in the potato plant: Its identification and changes in endogenous levels as influenced by temperature and photoperiod. Plant Physiology, 62, 438–442.

McCauley, A., Jones, C., & Jacobsen, J. (2011). Plant nutrient functions and deficiency and toxicity symptoms.

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.

Pérez, A., Nápoles, L., Carvajal, C., Hernandez, M., & Lorenzo, J. C. (2004). Effect of sucrose, inorganic salts, inositol, and thiamine on protease excretion during pineapple culture in temporary immersion bioreactors. Vitro Cellular & Developmental Biology – Plant, 40(3), 311–316.

Rahmani, A. H., Shabrmi, F. M., & Aly, S. M. (2014). Active ingredients of ginger as potential candidates in the prevention and treatment of diseases via modulation of biological activities. International Journal of Physiology, Pathophysiology and Pharmacology, 6(2), 125–136.

Roumeliotis, E., Visser, R. G. F., & Bachem, C. W. B. (2012). A crosstalk of auxin and GA during tuber development. Plant Signaling & Behavior, 7(10), 1360–1363.

Rout, G. R., Palai, S. K., Samantaray, S., & Das, P. (2001). Effect of growth regulator and culture conditions on shoot multiplication and rhizome formation in ginger (Zingiber officinale Rosc.) in vitro. Vitro Cellular & Developmental Biology – Plant, 37(6), 814–819.

Singh, T. D., Chakpram, L., & Devi, H. S. (2013). Induction of in vitro microrhizomes using silver nitrate in Zingiber officinale Rosc. var. Baishey and Nadia. Indian Journal of Biotechnology, 13, 256–262.

Swarnathilaka, D. B. R., Kottearachchi, N. S., & Weerakkody, W. J. S. K. (2016). Factors affecting on induction of microrhizomes in ginger (Zingiber officinale Rosc.), cultivar Local from Sri Lanka. British Biotechnology Journal, 12(2), 1–7.

Wilson, L. A. (1977). Root crops. In P. de T. Alvim & T. T. Kozlowski (Eds.), Ecophysiology of tropical crops (pp. 219–220). Academic Press. 055650-2.50012-5

Xu, X., Lammeren, A. A. M., Vermeer, E., & Vreugdenhil, D. (1998). The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiology, 117, 575–584.

Yang, S. F., & Hoffman, N. E. (1984). Ethylene biosynthesis and its regulation in higher plants. Annual Review of Plant Physiology, 35, 155–189.

Zhao, D. W. (2002). High quality and production of ginger-theory and technology. China Agricultural Publishing Company.