Effects of Some Plant Extracts, Bioagents, and Organic Compounds on Botrytis and Sclerotinia Molds

Hala Abdel Wahab, Ahmed Malek, Mohamed Ghobara


Botrytis cinerea and Sclerotinia sclerotiorum are necrotrophic fungi and are closely related pathogenic fungi that infect hundreds of plant species worldwide. In this study, the natural botryticidal/scleroticidal efficacy of some plant extracts, bioagents, and organic compounds known to possess antifungal activity was evaluated. Pathogenicity tests of the fungal molds showed virulence divergence, depending on the isolate and host plant. All B. cinerea isolates, except the BF isolate that infected only broad bean leaves, demonstrated ability to infect detached lettuce and broad bean leaves. Moreover, all Sclerotinia sclerotiorum isolates, except for SSP, demonstrated ability to infect the two plant species, whereas the SSB isolate did not cause any infection in broad bean leaves. The efficacies of Moringa oleifera (Mor), Cinnamomum zeylanicum (Cin), amino acid derivatives (Aad), Trichoderma harzianum (TH), Cactus spp. (Agr), and Bacillus subtilis (BS) were tested either in vitro or in vivo against the highly virulent isolates of the two pathogenic fungi. The efficacy of most potential biofungicides was consistent in vitro as well as in vivo, and the inhibitory efficacy of TH, BS, Cin, Mor, and Aad treatments was significantly high against Botrytis cinerea and Sclerotinia sclerotiorum in vitro and ranged from 62% to 100%, depending on the isolate. In addition, BS, Aad, TH, and Mor treatments had significant inhibitory effects ranging from 53% to 100% against most of the isolates on lettuce leaves. The Agr and Cin treatments exhibited low or no inhibitory effects against many isolates in vivo, and they reduced the mold infection caused by only BCC and SSB isolates. Most of the tested potential biofungicide treatments tended to reduce mold infections, and some of them, such as Cin, exhibited a higher inhibitory effect in vitro than the others. Real-time PCR was conducted for some symptomatic/asymptomatic samples, and the results showed either consistent molecular/symptomatic patterns or latency of B. cinerea. The results confirmed the suitability of the studied natural compounds as effective biofungicides, and they could be the best choice to safely control the most destructive fungal molds.


biocontrol; Botrytis cinerea; biofungicides; microbial pesticides; Sclerotinia sclerotiorum

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Abbott, W. S. (1987). A method of computing the effectiveness of an insecticide. Journal of the American Mosquito Control Association, 3(2), 302–303.

Abdel Wahab, H., & Helal, N. S. (2013). Evaluation of pre-harvest bioagent applications for both production and biological control of onion and strawberry plants under natural Botrytis infections. African Journal of Plant Science and Biotechnology, 7(1), 64–69.

Aboelghar, M., Moustafa, M. S., Ali, A. M., & Abdel Wahab, H. (2019). Hyperspectral analysis of Botrytis cinerea infected lettuce. International Journal of Agriculture and Environmental Research, 5(4), 26–42.

Alavanja, M. C. R., Hoppin, J. A., & Kamel, F. (2004). Health effects of chronic pesticide exposure: Cancer and neurotoxicity. Annual Review of Public Health, 25(4), 155–197. https://doi.org/10.1146/annurev.publhealth.25.101802.123020

Boland, G. J., & Hall, R. (1994). Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology, 16(2), 93–108. https://doi.org/10.1080/07060669409500766

Bonaterra, A., Camps, J., & Montesinos, E. (2005). Osmotically induced trehalose and glycine betaine accumulation improves tolerance to desiccation, survival and efficacy of the postharvest biocontrol agent Pantoea agglomerans EPS125. FEMS Microbiology Letters, 250(1), 1–8. https://doi.org/10.1016/j.femsle.2005.06.028

Brozová, J. (2002). Exploitation of the mycoparasitic fungus Pythium oligandrum in plant protection. Plant Protection Science, 38(1), 29–35. https://doi.org/10.17221/4818-PPS

Calvo-Garrido, C., Roudet, J., Aveline, N., Davidou, L., Dupin, S., & Fermaud, M. (2019). Microbial antagonism toward Botrytis bunch rot of grapes in multiple field tests using one Bacillus ginsengihumi strain and formulated biological control products. Frontiers in Plant Science, 10(2), Article 105. https://doi.org/10.3389/fpls.2019.00105

Camele, I., Altieri, L., Martino, L. D., Feo, V., Mancini, E., & Rana, G. L. (2012). In vitro control of post-harvest fruit rot fungi by some plant essential oil components. International Journal of Molecular Science, 13(2), 2290–2300. https://doi.org/10.3390/ijms13022290

Chunmei, W., Jie, Z., Hao, C., Yongjian, F., & Zhiqi, S. (2010). Antifungal activity of eugenol against Botrytis cinerea. Tropical Plant Pathology, 35(3), 137–143. https://doi.org/10.1590/S1982-56762010000300001

Clarkson, J. P., Staveley, J., Phelps, K., Young, C. S., & Whipps, J. M. (2003). Ascospore release and survival in Sclerotinia sclerotiorum. Mycological Reserch, 107(2), 213–222. https://doi.org/10.1017/S0953756203007159

Elad, Y. (2000a). Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Protection, 19(8), 709–714. https://doi.org/10.1016/S0261-2194(00)00094-6

Elad, Y. (2000b). Trichoderma harzianum T39. Preparation for biocontrol of plant diseases-control of Botrytis cinerea, Sclerotinia sclerotiorum and Cladosporium fulvum. Biocontrol Science and Technology, 10(4), 499–507. https://doi.org/10.1080/09583150050115089

Epstein, L. (2014). Fifty years since Silent Spring. Annual Review of Phytopathology, 52(6), 377–402. https://doi.org/10.1146/annurev-phyto-102313-045900

Fan, X., Zhang, J., Yang, L., Wu, M., Chen, W., & Li, G. (2015). Development of PCR-based assays for detecting and differentiating three species of Botrytis infecting broad bean. Plant Disease, 99(5), 691–698. https://doi.org/10.1094/PDIS-07-14-0701-RE

Fiume, F., & Fiume, G. (2005). Biological control of Botrytis gray mould and Sclerotinia drop in lettuce. Communications in Agricultural and Applied Biological Sciences, 70(3), 157–168.

Floch, G. L., Rey, P., Déniel, F., Benhamou, N., Picard, K., & Tirilly, Y. (2003). Enhancement of development and induction of resistance in tomato plants by the antagonist, Pythium oligandrum. Agronomie, 23(5-6), 455–460. https://doi.org/10.1051/agro:2003018

Food and Agriculture Organization of the United Nations. (2020). Integrated pest management (IPM). http://www.fao.org/agriculture/crops/thematic-sitemap/theme/spi/scpi-home/ managing-ecosystems/integrated-pest-management/en/

Haidar, R., Fermaud, M., Calvo-Garrido, C., Roudet, J., & Deschamps, A. (2016). Modes of action for biological control of Botrytis cinerea by antagonistic bacteria. Phytopathologia Mediterranea, 55(3), 13–34. https://doi.org/10.14601/Phytopathol_Mediterr-18079

Marín, A., Chafer, M., Atares, L., Chiralt, A., Torres, R., Usall, J., & Teixidó, N. (2016). Effect of different coating-forming agents on the efficacy of the biocontrol agent Candida sake CPA-1 for control of Botrytis cinerea on grapes. Biological Control, 96(5), 108–119. https://doi.org/10.1016/j.biocontrol.2016.02.012

Martinez-Romero, D., Serrano, M., Bailen, G., Guillen, F., Zapata, P. J., Valverde, J. M., Castillo, S., Fuentes, M., & Valero, D. (2008). The use of a natural fungicide as an alternative to preharvest synthetic fungicide treatments to control lettuce deterioration during postharvest storage. Postharvest Biological Technology, 47(1), 54–60. https://doi.org/10.1016/j.postharvbio.2007.05.020

Nicot, P. C., Stewart, A., Bardin, M., & Elad, Y. (2016). Biological control and biopesticide suppression of Botrytis-incited diseases. In S. Fillinger & Y. Elad (Eds.), Botrytis – the fungus, the pathogen and its management in agricultural systems (pp. 165–187). Springer International Publishing. https://doi.org/10.1007/978-3-319-23371-0_9

Passera, A., Venturini, G., Battelli, G., Casati, P., Penaca, F., Quaglino, F., & Bianco, P. A. (2017). Competition assays revealed Paenibacillus pasadenensis strain R16 as a novel antifungal agent. Microbiological Research, 198(5), 16–26. https://doi.org/10.1016/j.micres.2017.02.001

Rabeendran, N., Jones, E. E., Moot, D. J., & Stewart, A. (2006). Biocontrol of Sclerotinia lettuce drop by Coniothyrium minitans and Trichoderma hamatum. Biological Control, 39(3), 352–362. https://doi.org/10.1016/j.biocontrol.2006.06.004

SAS. (2006). Statistical Analysis System, SAS user’s guide: Statistics. SAS Institute.

SIBAT. (1993). Organic pest control in rice, corn and vegetables. Techno-Series 1.

Subbarao, K. V. (1998). Progress toward integrated management of lettuce drop. Plant Disease, 82(10), 1068–1078. https://doi.org/10.1094/PDIS.1998.82.10.1068

Sylla, J., Alsanius, B. W., Kruger, E., & Wohanka, W. (2015). Control of Botrytis cinerea in strawberries by biological control agents applied as single or combined treatments. European Journal of Plant Pathology, 143(3), 461–471. https://doi.org/10.1007/s10658- 015-0698-4

Williamson, B., Tudzynski, B., Tudzynski, P., & Kan, J. A. (2007). Botrytis cinerea: The cause of grey mould disease. Molecular Plant Pathology, 8(5), 561–580. https://doi.org/10.1111/j.1364-3703.2007.00417.x

Zhao, J., Bi, Q., Wu, J., Lu, F., Han, X., & Wang, W. (2019). Occurrence and management of fungicide resistance in Botrytis cinerea on tomato from greenhouses in Hebei, China. Journal of Phytopathology, 167(7–8), 413–421. https://doi.org/10.1111/jph.12812