Changes in the chlorophyll content in stored lettuce Lactuca sativa L. after pre-harvest foliar application of CaCl2

Irena Perucka, Katarzyna Olszówka, Barbara Chilczuk

Abstract


The aim of the present investigations was to determine the cumulative effect of foliar treatment of lettuce plants with a CaCl2 solution and the cold-storage period on the level of chlorophylls in the leaves of lettuce cv. ،Omega٫ (Lactuca sativa L.). Plants were grown in a pot experiment conducted in the greenhouse of the Department of Cultivation and Fertilisation of Horticultural Plants, University of Life Sciences in Lublin. During the growing period, 10 and 20 days before harvest, the plants received foliar application of CaCl2 at the concentrations of 0.1 M and 0.2 M. Control plants were sprayed with water. Some plants were analysed immediately after the harvest; other specimens were cold-stored at a temperature of 4oC for 7 and 14 days. Whole leaves and blades without the midrib were analysed. The results obtained indicated that the foliar application of 0.2M CaCl2 in lettuce resulted in a decrease in the level of chlorophylls in fresh plants, compared with the control. A beneficial cumulative effect of the CaCl2 application and storage period on the chlorophyll level in lettuce leaves was observed in the leaf blades of plants after foliar treatment with the 0.1M CaCl2.solution and cold-stored for 7 and 14 days, in which in- creased levels of chlorophyll “a” and “b” and total chlorophyll, compared with the control, were found.

Keywords


Lactuca sativa L.; foliar CaCl2 application; chlorophylls; cold storage

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References


Serafini M, Bugianesi R, Salucci M, Azzini E, Raguzzini A, Maiani G. Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa ) on plasma total antioxidant capacity and antioxidant levels in human subjects. Brit J Nutit. 2002; 88: 615–623. http://dx.doi.org/10.1079/BJN2002722.

Nicolle C, Carnat A, Fraisse D, Lamaison J, Rock E, Michel H, Amouroux P, Remesy Ch. Characterization and variation of antioxidant micronutrients in lettuce (Lactuca sativa folium). J Sci Food Agric. 2004; 84: 2061–2069. http://dx.doi.org/10.1002/jfsa.1916

Hamułka J, Koczara J, Gronek M. Lutein content of selected Polish foods and estimation of its intake. Pol. J Food Nutr Sci. 2005: 14/55 (2): 201–206.

Kenny O, O’Beirne D. The effects of washing treatment on antioxidant retention in ready-to-use iceberg lettuce. J Food Sci Tech. 2009: 44: 1146–1156. http://dx.doi.org/10.1111/j.1365-2621.2009.01935.x.

Murillo E, Melendez-Martnez AJ, Portugal F. Screening of vegetables and fruits from Panama for rich sources of lutein and zeaxanthin. Food Chem. 2010; 122: 167–172. http://dx.doi.org/10.1016/j.foodchem.2010.02.034.

Perucka I, Olszówka K. Efekt of foliar calcium chloride treatment on the level of chlorogenic acid, β-carotene, lutein and tocopherols in lettuce (Lactuca sativa L.). Acta Agrobot. 2011; 64(1): 65–72.

Perucka I, Olszówka K. Accumulation of potassium, magnesium, calcium in fresh and cold stored leaves of lettuce (Lactuca sativa L.) after CaCl2 foliar treatment before harvest. J Elem. 2011: 445–454. http://dx.doi.org/10.5601/jelem.2011.16.3.09.

Degl’Innocenti E, Guidi L, Pardossi A, Tognoni F. Biochemical Study of leaf browning in minimally processed leaves of lettuce (Lactuca sativa L. Var. Acephala). J Agric Food Chem. 2005; 53: 9980–9984. http://dx.doi.org/10.1021/jf050927o.

Ferrante A, Martinetti L, Maggiore T. Biochemical changes in cut vs. intact lamb’s lettuce (Valerianella oliatoria) leaves during storage. Food Sci Technol. 2009; 44: 1050–1056. http://dx.doi.org/10.111/j.1365-2621.2008.01891.x

Ferrante A, Incrocci L, Maggini R, Serra G, Tognon E. Colour changes of fresh-cut leafy vegetables during storage. J Food Agric. Environ. 2004; 2: 40–44.

Ferrante A, Maggiore T. Chlorophyll a fluorescence measurements to evaluate storage time and temperature of Valeriana leafy vegetables. Postharvest Biol Technol. 2007; 45: 73–80. http://dx.doi.org/10.1016/j.postbarvbio.2007.02.003

Ferguson B. Calcium in plant senescence and fruit ripening. Plant Cell and Environment. 1984; 7: 477-489.

Ferguson BI, Watkins ChB, Harman JE. Inhibition by Calcium of senescence of detached cucumber cotyledons. Plant Physiol. 1983; 71: 182–186.

Luna-Guzman I, Cantwell M, Berrett DM. Fresh-cut cantaloupe : effects of CaCl2 dips and heat treatments on firmness and metabolic activity. Post Biol Tech. 1999; 17: 201–213.

Caldwell ChR, Britz SJ. Effect of supplemental ultraviolet radiation on the carotenoid and chlorophyll composition of greenhouse-grown leaf lettuce (Lactuca sativa L.) culivars. J Food Comp Anal. 2006; 19: 637–644. http://dx.doi.org/10.1016/j.jfca.2005.12.016

Degl’Innocenti E, Pardossi A, Tognoni F, Guidi L. Physiological basis of sensivity to enzymatic browning in lettuce, escarole and rocket salad when stored as fresh-cut products. Food Chem. 2007; 104: 209–215. http://dx.doi.org/10.1016/j.foodchem.2006.11.026

Perucka I, Sałata A, Bojanowska M. Zawartość barwników chlorofilowych I antocyjanowych w ogonkach liściowych wybranych odmian rabarbaru (Rheum rhaponticum L.). Ann UMCS sec. EEE IX smp. 2001: 195–201.