Uptake and transport of iron ions (Fe+2, Fe+3) supplied to roots or leaves in spinach (Spinacia oleracea L.) plants growing under different light conditions
Abstract
In experiments carried out in a phytotron using aqueous cultures, there was investigated the effect of root or foliar application of different types of iron salts on spinach plant productivity, leaf and root iron content as well as the rate of transport of iron from the roots to the leaves. Plants were grown in Hoagland's solution with a single concentration at two fluorescent light intensities: 290 and 95 µmol × m-2 × s-1 PAR. To fertilize the plants, iron was supplied at a dose of 25 mg Fe in the nutrient solution or as foliar sprays using the following salts: 1 – Fe 0; 2 – FeCl2 × 4H2O; 3 – FeCl3 × 4H2O; 4 – FeSO4 × 7H2O; 5 – Fe2(SO4)3 × nH2O; 6 – Fe-Cit.
The obtained results showed that the productivity of spinach plants treated with FeCl2 and FeSO4 using foliar sprays and of those fed with Fe-citrate (Fe-Cit) through the roots was significantly higher than in the case of the other salts used. Root application of the salts used had a significant effect on root iron content, whereas their foliar application significantly affected leaf iron content. In this respect, ferrous salts were generally the most beneficial, while ferric salts were the least beneficial. The rate of transport of iron to the leaves, irrespective of the method of its application, was clearly higher for ferrous salts and Fe-Cit than for ferric salts. The free proline content in the leaves of plants not fertilized with Fe was 2–4 times lower than in plants supplied with this nutrient. An irradiance of 290 µmol × m-2 × s-1 had a positive effect on plant productivity and root Fe content.
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Alvarez-Fernández A., Paniagua P., Abadia J., Abadia A. 2003. Effects of Fe deficiency chlorosis on yield and fruit quality in peach (Prunus persica L.
Batsch). J. Agric. Food Chem. 51: 5738-5744. http://dx.doi.org/10.1021/jf034402c
Baligar V.C., Schaffert R.E., Das Santos H.L., Pitta G.V.E., Bahia Filho A.F. 1993. Growth and nutrient uptake parameters in sorghum as influenced by aluminium. Agron. J. 85: 1068–1074. http://dx.doi.org/10.2134/agronj1993.00021962008500050021x
Bates L.S., Waldren R.R., Teare J.D. 1973. Rapid determination of free proline for water – stress studies. Plant Soil, 39: 205–207. http://dx.doi.org/10.1007/BF00018060
Bienfait H.F., Bimo R.J., Bliek A.M. Duivenvoorden J.F., Fontaine J.M. 1983. Characterization of ferric reducing activity in roots of Fe-deficient Phaseolus vulgaris. Physiol. Plant. 59: 196–202. http://dx.doi.org/10.1111/j.1399-3054.1983.tb00757.x
Binzel M.L., Hasegawa P.M., Rhodes D., Handa S., Handa A.K., Bressan R.A. 1987. Solute accumulation in tobacco cells adapted to NaCl. Plant Physiology, 84: 1408–1415. http://dx.doi.org/10.1104/pp.84.4.1408
Borowski E., Michałek S. 2010. The effects of foliar fertilization of French bean with iron salts and urea on some physiological processes in plants relative to iron uptake and translocation in leaves. Acta. Scient. Pol. Hort. Cult. 10: 183–193.
Brown J.C., Jolley V.D. 1986. An evaluation of concepts related to iron deficiency chlorosis. J. Plant. Nutr. 9: 175–182. http://dx.doi.org/10.1080/01904168609363435
Brüggeman W., Mass-Kantel K., Moog P.R. 1993. Iron uptake by leaf mesophyll cells: the role of the plasma membrane – bound ferric – chelate reductase. Planta, 190: 151–155. http://dx.doi.org/10.1007/BF00196606
Cacmak I., Wetering D.A.M., Marschner H., Bienfait H.F. 1987. Involvement of superoxide radical in extracellular ferric reduction by iron – deficient bean roots. Plant Physiol. 85: 310–314. http://dx.doi.org/10.1104/pp.85.1.310
Fernandez V., Ebert G., Winkelmann G. 2005. The use of microbial siderophores for foliar iron application studies. Plant and Soil, 272: 245–252. http://dx.doi.org/10.1007/s11104-004-5212-2
Fernandez V, Del Rio V., Abadia J., Abadia A. 2006. Foliar iron fertilization of peach (Prunus persica (L.) Batsch): Effect of iron compounds, surfactants and other adjuvants. Plant Soil, 289: 239–252. http://dx.doi.org/10.1007/s11104-006-9132-1
Kannan S., Wittwer S.H. 1965. Effects of chelation and urea on iron absorption by intact leaves and enzymically isolated leaf cells. Plant Physiol. 40: 12–18.
Ketchum R.E.B., Warren R.C., Klima L.J., Lopez-Gutierrez F., Nabors M.W. 1991. The mechanism and regulation of proline accumulation in suspension cultures of the halophytic grass Distichlis spicata L. Plant Physiology, 137: 368–374. http://dx.doi.org/10.1016/S0176-1617(11)80147-1
Larbi A., Morales F., López –Millan A.F., Gogorcena Y., Abadia A., Moog P.R., Abadia J. 2001. Technical advance: reduction of Fe (III)-chelates by mesophyll leaf disks of sugar beet. Multi – component origin and effects of Fe deficiency. Plant Cell Physiol. 42: 94–105. http://dx.doi.org/10.1093/pcp/pce012
Longnecker N., Weich R.M. 1990. Accumulation of apoplastic iron in plant roots. Plant Physiol. 92: 17–22. http://dx.doi.org/10.1104/pp.92.1.17
Mortvedt J.J. 1991. Correcting iron deficiencies in annual and perennial plants: Present technologies and future prospects. Plant and Soil, 130: 273–279. http://dx.doi.org/10.1007/BF00011883
Pardha Saradhi P., Alia, Vani B. 1993. Inhibition of mitochondrial electron transport is the prime cause behind proline accumulation during mineral deficiency in Oryza sativa. Plant and Soil, 155/156: 465–468.
Reed D.W., Lyons C.G., McEachern G.R. 1988. Field evaluation of inorganic and chelated iron fertilizers as foliar sprays and soil application. J. Plant Nutr. 11: 1369–1378. http://dx.doi.org/10.1080/01904168809363894
Rombola A.D., Brüggeman W., Togliavini M., Marangoni B., Moog P.R. 2000. Iron source affects iron reduction and re-greening of kiwifruit (Actinidia deliciosa) leaves. J. Plant. Nutr. 23: 1751–1765. http://dx.doi.org/10.1080/01904160009382139
Rombola A.D., Brüggeman W., López-Millán A.F., Togliavini M., Abadia J., Maragoni B., Moog P.R. 2002. Biochemical responses to iron deficiency in kiwifruit (Actinidia deliciosa). Tree Physiol. 22: 869–875. http://dx.doi.org/10.1093/treephys/22.12.869
Römheld V., Marschner H. 1986. Mobilization of iron in the rhizosphere of different plant species. Adv. Plant Nutr. 2: 155–204.
Sieńko M.J., Plane R.A. 1980. Chemia podstawy i zastosowanie. Chemistry principles and applications. Wyd. Nauk. Techn. Warszawa. (in Polish)
Sijmons P.C., Briel W., Bienfait H.F. 1984. Cytosolic NADPH is the electron donor for extracellular Fe-III reduction in iron – deficient bean roots. Plant Physiol. 75: 219–221.
Silver J. 1993. Introduction to Fe chemistry. In “The chemistry of iron”, Glasgow, UK: Blackie Academic and Professional, Chapman and Hall.
DOI: https://doi.org/10.5586/aa.2013.021
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