Analiza pigmentów antrachinonowych Pyrenophora triticirepentis (Died.) Drechs. [Analysis of anthraquinone pigments of Pyrenophora tritici-repentis (Died.) Drechs.]

Abstract

Pyrenophora tritici-repentis, anamorph Drechslera tritici-repentis, strains used in this study represented three phenotypic classes of this fungus: the wild type, strains unable to produce melanins – mel(-), and intermediate strains. Using an HPLC system with a reversed phase C₁₈ column synthesis of anthraquinone pigments by strains belonging to all three classes could be detected. According to the ¹H NMR spectra analysis the major compounds produced by P.tritici-repentis are catenarin, helminthosporin, islandicin, emodin, ω-hydroxy-islandicin, ω-hydroxy-catenarin and ω-hydroxy-helminthosporin. However, amounts of these compounds produced by the wild type were up to 350 times lower than those observed for the mel(-) forms. Some differences in profiles of these compounds produced by different strains were also observed.